FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5426
Sequences flagged as poor quality0
Sequence length151
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC56110.339107998525618No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2224.0914117213416885No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG420.7740508661997788No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG290.5344636933284188No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.44231478068558794No Hit
ATTGCGCAATGTAGTACCGGGGGAGCCTGGAGGAACAGGTAGGTTTCGAG180.3317360855141909No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG160.2948765204570586No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC120.22115739034279397No Hit
ATTGCGCAATGACGGATGGGGGGCAGAGTACAGAAAGGGTGCATTTTCAC110.2027276078142278No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC100.18429782528566163No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT90.16586804275709546No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTG90.16586804275709546No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC90.16586804275709546No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.16586804275709546No Hit
ATTGCGCAATGTTCAGTGGGGGATGCCGAGCAGCGTGGAGAATCTGTTGA80.1474382602285293No Hit
ATTGCGCAATCTGGCTCTTATACACCTGTCTCTTATACACATCTCCGAGC70.12900847769996315No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT70.12900847769996315No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC60.11057869517139698No Hit
ATTGCGCAATGTGTGGGTAGGGGTAGACACGCTGTTAAAGAAGCAATTGT60.11057869517139698No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.11057869517139698No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.11057869517139698No Hit
ATTGCGCAATATGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC60.11057869517139698No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG60.11057869517139698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT60.11057869517139698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG60.11057869517139698No Hit
ATTGCGCAATATGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC60.11057869517139698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)