FastQCFastQC Report
Tue 7 May 2024
EGAF00007822261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6347
Total Bases958.3 kbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2173.418938080983142No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1392.190011028832519No Hit
ATTGCGCAATGATGATGCCGGGATGTGAAAGGTTAAAGTTTATTTTCTGC1261.9851898534740822No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA731.150149677012762No Hit
ATTGCGCAATGTTAGTGGCGGGGCCTTAGTTCTCTTTCCCGTCTTGCAAG540.8507956514888924No Hit
ATTGCGCAATGTACTGATGGGGAGGATGAAAGCAGGAAGGGCACTAGAAA540.8507956514888924No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG490.772018276351032No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG370.582952576020167No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA310.4884197258547345No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTC280.44115330077201825No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG220.34662045060658575No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG160.2520876004411533No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATGG140.22057665038600913No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.22057665038600913No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT140.22057665038600913No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT110.17331022530329288No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT110.17331022530329288No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA90.14179927524814873No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAT90.14179927524814873No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA90.14179927524814873No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAGCG80.12604380022057665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT80.12604380022057665No Hit
ATTGCGCAATGGGGAGGGGGGGGAGGAAAAGAAAAGAAAAAGACGGAGAG80.12604380022057665No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG80.12604380022057665No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.11028832519300456No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAGCG70.11028832519300456No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC70.11028832519300456No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.11028832519300456No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATGG70.11028832519300456No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA70.11028832519300456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)