FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6347
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTA3185.010241058767922No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2874.521821332913188No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1872.946273830155979No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.9610839766818969No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTA470.7405073262958879No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.7089963762407437No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGG440.6932409012131715No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCG370.582952576020167No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG360.5671971009925949No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTC360.5671971009925949No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC310.4884197258547345No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.4096423507168741No Hit
GCTATCTTAAGAAACTGTCTTCTTTCCTTTGGCTTACAGTCTACACCATA200.3151095005514416No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.2993540255238695No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG190.2993540255238695No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA180.28359855049629745No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG170.2678430754687254No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTG170.2678430754687254No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTCGG160.2520876004411533No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA150.2363321254135812No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.2363321254135812No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC130.20482117535843705No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.20482117535843705No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC130.20482117535843705No Hit
ATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGG120.18906570033086498No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA120.18906570033086498No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG120.18906570033086498No Hit
GTCTACACCATAAAAAATTGAATATAATACAAAACAATTTACTATCTATC120.18906570033086498No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG110.17331022530329288No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT100.1575547502757208No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA100.1575547502757208No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.1575547502757208No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.1575547502757208No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA100.1575547502757208No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTAGATCTCGG90.14179927524814873No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGTCGCC90.14179927524814873Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAA80.12604380022057665No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC80.12604380022057665No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC80.12604380022057665No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT70.11028832519300456No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA70.11028832519300456No Hit
GTCTTCTTTCCTTTGGCTTACAGTCTACACCATAAAAAATTGAATATAAT70.11028832519300456No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA70.11028832519300456No Hit
GTAATAAAGGCCATAAAAATAACAGCTCTGCCGTATTGCACCGATAAATT70.11028832519300456No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGGGGCC70.11028832519300456No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT70.11028832519300456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG70.11028832519300456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA70.11028832519300456No Hit
TAATTATACAGCTTTAATTTCTTGGGCACTTTTGATCATATAAATATATA70.11028832519300456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)