FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9451
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG2732.8885832187070153No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2392.5288329277325152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1942.052692836736853No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCT1481.5659718548301769No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1431.5130674002751032No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACAT730.7724050365040735No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG660.6983388001269707No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC520.5502063273727648No Hit
ATTGCGCAATGTCTATCCTGGGAAGCGTGTTGATGAGCTCAAGGCCAAGG490.5184636546397207No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT400.42323563644058826No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG390.41265474552957354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG390.41265474552957354No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC380.40207385461855893No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAT360.3809120727965295No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT350.3703311818855148No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT300.31742672733044125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT290.30684583641942653No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC270.2856840545973971No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCT250.26452227277536766No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.26452227277536766No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.21161781822029413No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC190.20103692730927947No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA180.19045603639826475No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA180.19045603639826475No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.19045603639826475No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.17987514548725003No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAA170.17987514548725003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG170.17987514548725003No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGG160.16929425457623534No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA150.15871336366522062No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG150.15871336366522062No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1481324727542059No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAGCG140.1481324727542059No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACA130.1375515818431912No Hit
ATTGCGCAATGAACACGACGGGCGAGACCATCCTGGCTAACATGGTGAAA120.12697069093217647No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATGG120.12697069093217647No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG120.12697069093217647No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG110.11638980002116178No Hit
ATTGCGCAATGAGGTGAGGGGGAAAAGCGCAAGCTGGTGATCCCATCCGA110.11638980002116178No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT100.10580890911014706No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.10580890911014706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT100.10580890911014706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)