FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9451
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTA5035.322188128240398No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3023.195429055126442No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1942.052692836736853No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1201.269706909321765No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT951.0051846365463972No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCG870.9205375092582796No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTA820.867633054703206No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGG500.5290445455507353No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG460.48672098190667656No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA410.433816527351603No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.42323563644058826No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG390.41265474552957354No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG370.3914929637075442No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTG310.32800761824145597No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTC300.31742672733044125No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.2751031636863824No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.2751031636863824No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC250.26452227277536766No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC230.24336049095333828No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC230.24336049095333828No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA220.23277960004232356No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTT210.22219870913130885No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG190.20103692730927947No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC190.20103692730927947No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG170.17987514548725003No Hit
ATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGG170.17987514548725003No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTC170.17987514548725003No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA170.17987514548725003No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.17987514548725003No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACG160.16929425457623534No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA150.15871336366522062No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA150.15871336366522062No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG140.1481324727542059No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTCGG130.1375515818431912No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.1375515818431912No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT130.1375515818431912No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGA120.12697069093217647No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.12697069093217647No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.12697069093217647No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGTCGCC120.12697069093217647Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC120.12697069093217647No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA110.11638980002116178No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA110.11638980002116178No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGA110.11638980002116178No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATGTCGG110.11638980002116178No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATTT100.10580890911014706No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA100.10580890911014706No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA100.10580890911014706No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA100.10580890911014706No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTC100.10580890911014706No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.10580890911014706No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA100.10580890911014706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)