FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11081
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTA6405.775652016965978No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2892.608067863911199No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2222.0034292933850737No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1371.2363505098817795No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCG1030.929518996480462No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.82122552116235No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTA820.7400054146737659No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG520.4692717263784857No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGG520.4692717263784857No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTG510.46024727010197636No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTC500.451222813825467No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.4331739012724483No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.3790271636133923No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC380.34292933850735496No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC330.29780705712480826No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.28878260084829893No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA310.27975814457178955No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC280.2526847757422615No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG280.2526847757422615No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA270.24366031946575217No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC250.2256114069127335No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGTCGCC230.20756249435971483Illumina RNA PCR Primer (95% over 22bp)
ATATTATACTTAACCAAAGGAGTAGTGTAAATAGTTTACGGTTTGGACTA230.20756249435971483No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.1804891255301868No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTT200.1804891255301868No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG180.16244021297716812No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.16244021297716812No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.16244021297716812No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.1534157567006588No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.1534157567006588No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA160.14439130042414947No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA160.14439130042414947No Hit
GGTTTCTCACCCGTCTTTCGCTACTCATGCCTGCATTCTCACTCGTCCAC150.1353668441476401No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA150.1353668441476401No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT150.1353668441476401No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.12634238787113075No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTAGATCTCGG140.12634238787113075No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA140.12634238787113075No Hit
ATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGG140.12634238787113075No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA140.12634238787113075No Hit
GTGTAAATAGTTTACGGTTTGGACTACTCAGGTAGCTAATCTGTTTCGCT130.11731793159462142No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA130.11731793159462142No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG130.11731793159462142No Hit
CATACAGCCTTTCGCTTTCCCTCTGATTTTATTTTCAGTATATTATACTT120.10829347531811208No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAG120.10829347531811208No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACG120.10829347531811208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)