FastQCFastQC Report
Tue 7 May 2024
EGAF00007822271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7891
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG2523.193511595488531No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2032.5725510074768723No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1882.382461031554936No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT1842.3317703713090863No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1381.7488277784818147No Hit
ATTGCGCAATGGGAGAGGTGGGAGAGAGAGATCTCAGTAGATCTAAGTCG750.9504498796096819No Hit
ATTGCGCAATGCCACAACAGGGATAGGGGTGTGGGTGGTAACGCGGGGAA610.7730325687492079No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG510.6463059181345837No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGC480.6082879229501964No Hit
ATTGCGCAATGTTCGGCGGGGGGGCCCCTCCTTGTCATGTCTGCCGGCAT470.595615257888734No Hit
ATTGCGCAATGGAAGGAGGGGGGTAACCAGTTGATATTCTGGTACCGGCT460.5829425928272716No Hit
ATTGCGCAATGTTTTCGCGGGGGGGCCCCTCCTTGTCATGTCTGCCGGCA460.5829425928272716No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAT340.43087061208972244No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.36750728678241035No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG280.35483462172094793No Hit
ATTGCGCAATGCTCGGGGAGGGGGGTAACCAGTTGATATTCTGGTACCGG270.3421619566594855No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA260.3294892915980231No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT240.3041439614750982No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC240.3041439614750982No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA230.2914712964136358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC220.27879863135217337No Hit
ATTGCGCAATGCGCAGGGTGGGGGGTAACCAGTTGATATTCTGGTACCGG210.26612596629071095No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG170.21543530604486122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT160.20276264098339883No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCACTCG160.20276264098339883No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA150.19008997592193638No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.19008997592193638No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC140.17741731086047396No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG140.17741731086047396No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT140.17741731086047396No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACG130.16474464579901155No Hit
ATTGCGCAATGATATCTTGGGGGAGGCCGGCATCGCCCTGCCCACGCAGG130.16474464579901155No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGC130.16474464579901155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC120.1520719807375491No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG120.1520719807375491No Hit
ATTGCGCAATGTTACTGGCGGGGGGGCCCCTCCTTGTCATGTCTGCCGGC120.1520719807375491No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.13939931567608668No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT110.13939931567608668No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC110.13939931567608668No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG100.12672665061462424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG100.12672665061462424No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC100.12672665061462424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC100.12672665061462424No Hit
ATTGCGCAATGTTTCTCTAGGGGTTGAGGCATGATGGTGAGCCCACGGTT100.12672665061462424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT90.11405398555316183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT90.11405398555316183No Hit
ATTGCGCAATGGGGGGAAGGGGATGGAGGAGGGAATCGTGCGACAATAGG90.11405398555316183No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.11405398555316183No Hit
ATTGCGCAATGTATGCTGCTGCTGTCTCTTATACACATCTCCGAGCCCAC80.10138132049169941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)