FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7891
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTA4195.309846660752756No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2713.434292231656318No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1762.230389050817387No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1081.368647826637942No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.9631225446711444No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTA660.8363958940565202No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCG570.7223419085033582No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.4815612723355722No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.4181979470282601No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA320.40552528196679766No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTG310.3928526169053352No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTC290.36750728678241035No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA280.35483462172094793No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG280.35483462172094793No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.35483462172094793No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG270.3421619566594855No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC270.3421619566594855No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGG270.3421619566594855No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA240.3041439614750982No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC230.2914712964136358No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG210.26612596629071095No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.2407806361677861No Hit
ATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGG180.22810797110632366No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.22810797110632366No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT160.20276264098339883No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG160.20276264098339883No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTT150.19008997592193638No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.19008997592193638No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC130.16474464579901155No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA130.16474464579901155No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTC130.16474464579901155No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGTCGCC130.16474464579901155Illumina RNA PCR Primer (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA120.1520719807375491No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG120.1520719807375491No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGA110.13939931567608668No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA110.13939931567608668No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.13939931567608668No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA110.13939931567608668No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA100.12672665061462424No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTAGATCTCGG100.12672665061462424No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC100.12672665061462424No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT100.12672665061462424No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11405398555316183No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGG90.11405398555316183No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA90.11405398555316183No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTCGG90.11405398555316183No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACG90.11405398555316183No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC90.11405398555316183No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA80.10138132049169941No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT80.10138132049169941No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGA80.10138132049169941No Hit
GTTTAGGGTACGGGCGGACCCGCCACCTCGCTCACGAAGCTTTTCTCGAC80.10138132049169941No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC80.10138132049169941No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG80.10138132049169941No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTAGATCTC80.10138132049169941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)