FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10979
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTA5224.754531378085436No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4333.9438928864195284No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2732.4865652609527276No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1561.4208944348301302No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.8926131705984152No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTA820.7468804080517352No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCG810.7377721103925676No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC560.5100646689133801No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA530.48273977593587764No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.48273977593587764No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGG490.4463065852992076No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTG430.3916567993442026No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG420.38254850168503507No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTC420.38254850168503507No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC410.3734402040258676No Hit
GTCCAGGTTCAACCGGATGCACTGCCTCAGGTCGGCGACCATGTCCTCGG350.31879041807086256No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.30057382275252753No Hit
GTTTATTATTGAGTTTCATATTTTATATTGTGTATTTTATATTTATAATT260.23681573913835502No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA250.22770744147918753No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC220.20038254850168502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.20038254850168502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.20038254850168502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.19127425084251753No Hit
TCCGAGAACTGGTCGATGAAGGCGGTGAGGTCCACCTCGTCCGTCTCCGC210.19127425084251753No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC200.18216595318335005No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTCGG200.18216595318335005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.18216595318335005No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTAGATCTCGG190.17305765552418254No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG190.17305765552418254No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.17305765552418254No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTT180.16394935786501502No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGTCGCC180.16394935786501502Illumina RNA PCR Primer (95% over 22bp)
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG170.1548410602058475No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA170.1548410602058475No Hit
ATCCTACACAAGCCACACCGAACACCAATACCAAACTATAGTAAAGGTCA170.1548410602058475No Hit
GTCCTAAAACAGGTAACTAATCTCACAATTAGTTGGGTTGCCCCATTCGG160.14573276254668002No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA160.14573276254668002No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC160.14573276254668002No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTC160.14573276254668002No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATGTCGG160.14573276254668002No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA150.13662446488751254No Hit
ATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGG150.13662446488751254No Hit
GTGCACGTCTGGGCGCACTCGAAGCAGGCCTGGATGCATTCGGCCAGTTT150.13662446488751254No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.12751616722834502No Hit
ATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGGGG140.12751616722834502No Hit
CCCCTGGACTCTGCGCCCCTCACCGCCACCACACCGCACGGGTGCACGAC140.12751616722834502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.11840786956917751No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACG130.11840786956917751No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGA130.11840786956917751No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTC130.11840786956917751No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT120.10929957191001002No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG120.10929957191001002No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA120.10929957191001002No Hit
GAGCAGGAGGGCTCGCGGATGACCACGCCGATCAGCTCGCGCAGGGTCTC110.10019127425084251No Hit
GCCTCAGGTCGGCGACCATGTCCTCGGCCAGGCAGGCGTCCGCGCAGGCG110.10019127425084251No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC110.10019127425084251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)