FastQCFastQC Report
Tue 7 May 2024
EGAF00007822275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12528
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG4283.4163473818646235No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCT3162.5223499361430397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2021.6123882503192848No Hit
ATTGCGCAATGCGCTGATTGGGAAGTTTCCAAGGGCGCATGGTGGATGCC1931.5405491698595148No Hit
ATTGCGCAATGTCACTCATGGGGGCAGTCCTGAGCTCGCAGTTCCAGGTT1230.9818007662835249No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1160.9259259259259258No Hit
ATTGCGCAATGCAGGACAGGGGCGGCGGAGCATGCGGATTAATTCGATGC1150.9179438058748404No Hit
ATTGCGCAATGAGAGGCCGGGGACCGCGTGGTGATTCCCTTCAACGTCTC1100.8780332056194126No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC990.7902298850574714No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGAT820.6545338441890166No Hit
ATTGCGCAATGACCACGAGGGGAGGGGAGCGTTCTGTAGGCTGATGAAGG720.5747126436781609No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAT620.49489144316730527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG510.407088122605364No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC490.3911238825031928No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC470.3751596424010217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT450.35919540229885055No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA420.33524904214559387No Hit
ATTGCGCAATGATATTGCTGGGGGTTTCCTACGCAACGTTCATCGGCGTA380.30332056194125157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.28735632183908044No Hit
ATTGCGCAATGCCGTTCCCGGGCAGGGCTCTGCTAAGTCGGCTTCAAGAC350.2793742017879949No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT330.26340996168582376No Hit
ATTGCGCAATGGAACCCTAGGGGGTCAAGCGAAAGAGGTTTATGGCGGAT320.2554278416347382No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCG320.2554278416347382No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA300.23946360153256704No Hit
ATTGCGCAATGCTGTGGGGGGTGGTCAAGCGAAAGAGGTTTATGGCGGAT280.2234993614303959No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT250.1995530012771392No Hit
ATTGCGCAATGAGCACCGTGGGGGATCAAGCGACTAAGTGCATACGGTGG250.1995530012771392No Hit
ATTGCGCAATGAACCGGGGGGGCAAGCGCATTAACAGGTGTGACGCAGGA240.19157088122605362No Hit
ATTGCGCAATGGGACCAGAGGGGTGCCTAAGCTACTAAGGGTATCTGGTG230.18358876117496806No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG220.1756066411238825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA220.1756066411238825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.1756066411238825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.1756066411238825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.15166028097062578No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGA180.14367816091954022No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.14367816091954022No Hit
ATTGCGCAATGGGTACATGGGGAAGTTTCCAAGGGCGCATGGTGGATGCC180.14367816091954022No Hit
ATTGCGCAATGCATACGGGGGGGTGCTGGTGTGAGGGGATCGGGGCCGCA170.13569604086845466No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT170.13569604086845466No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.1277139208173691No Hit
ATTGCGCAATGTACCAAAGGGGTGCCTAAGCTACTAAGGGTATCTGGTGG160.1277139208173691No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCT160.1277139208173691No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCGCG150.11973180076628352No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG150.11973180076628352No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT150.11973180076628352No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCT140.11174968071519795No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG140.11174968071519795No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG140.11174968071519795No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAGCG130.10376756066411238No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.10376756066411238No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC130.10376756066411238No Hit
ATTGCGCAATGATGTAATGGGGCAATAACACAACGAAATAAAAAATAAAA130.10376756066411238No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG130.10376756066411238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)