FastQCFastQC Report
Tue 7 May 2024
EGAF00007822276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12528
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTA8366.673052362707535No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1431.1414431673052363No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1391.109514687100894No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTA1050.8381226053639846No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.7902298850574714No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCG940.7503192848020434No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGG750.5986590038314177No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.590676883780332No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTG640.5108556832694764No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTC550.4390166028097063No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA470.3751596424010217No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG430.34323116219667943No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG390.3113026819923371No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.2793742017879949No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.2713920817369093No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC320.2554278416347382No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTT290.23148148148148145No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG280.2234993614303959No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.20753512132822477No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC230.18358876117496806No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTC220.1756066411238825No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.16762452107279693No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA210.16762452107279693No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.15964240102171137No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.15964240102171137No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTCGG180.14367816091954022No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.14367816091954022No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC170.13569604086845466No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG170.13569604086845466No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGA170.13569604086845466No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT160.1277139208173691No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.1277139208173691No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA160.1277139208173691No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT150.11973180076628352No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGTCGCC150.11973180076628352Illumina RNA PCR Primer (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTAGATCTC150.11973180076628352No Hit
GTCTATGTGTTCAACCGGGGGTCACACGACACGTCGTGCGGGGTTTCCCC150.11973180076628352No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACG150.11973180076628352No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA140.11174968071519795No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG130.10376756066411238No Hit
GTTTAGCCCCATCCGCGTTCGCTCGCCACTACTAACGGAATCACTTTTGT130.10376756066411238No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA130.10376756066411238No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATGTCGG130.10376756066411238No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTC130.10376756066411238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)