FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10577
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4544.292332419400586No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG4113.8857899215278433No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCT2792.6377989978254703No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2522.382528127068167No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.6901767987141912No Hit
ATTGCGCAATGCGATGGGAGGGGGGGGTCGAAGGAGGACCCGCCTTGTCG680.6429044152406164No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG610.5767230783776117No Hit
ATTGCGCAATGATGGTCATGGGGGTACAACAGTCTGCCAAGTTTACCATA600.5672686016828968No Hit
ATTGCGCAATGGTTCGGCGGGGGGGGTCGAAGGAGGACCCGCCTTGTCGT580.548359648293467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC440.4159969745674577No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAT420.3970880211780278No Hit
ATTGCGCAATGGCATTATCGGGGGGGGTCGAAGGAGGACCCGCCTTGTCG400.3781790677885979No Hit
ATTGCGCAATGAGTCTTAAGGGGGGGGTCGAAGGAGGACCCGCCTTGTCG390.36872459109388295No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCG390.36872459109388295No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA370.34981563770445306No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT340.3214522076203082No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT320.3025432542308783No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG320.3025432542308783No Hit
ATTGCGCAATGTGATCACTGGGATGAAGGGCGGTGACGACAAGGGCTCCA280.26472534745201853No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT250.23636191736787368No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG250.23636191736787368No Hit
ATTGCGCAATGCGGACACTGGGGTAAACAAAACTGACGCAGCATTGTTAC230.21745296397844377No Hit
ATTGCGCAATGTTTTCGGGGGGGGCAAAAGCTCAAATTTGAAATCTGGCA210.1985440105890139No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC210.1985440105890139No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.18908953389429894No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA200.18908953389429894No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATGG190.179635057199584No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG180.17018058050486906No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAT170.1607261038101541No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.15127162711543915No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.1418171504207242No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC150.1418171504207242No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.1418171504207242No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAGCG150.1418171504207242No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.13236267372600927No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC140.13236267372600927No Hit
ATTGCGCAATGTTTTCGGGGGGGGGCAAAAGCTCAAATTTGAAATCTGGC130.12290819703129433No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC130.12290819703129433No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT130.12290819703129433No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.12290819703129433No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC120.11345372033657937No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT120.11345372033657937No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATGT120.11345372033657937No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT110.10399924364186443No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA110.10399924364186443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.10399924364186443No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCGCG110.10399924364186443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG110.10399924364186443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)