FastQCFastQC Report
Tue 7 May 2024
EGAF00007822279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5478
Total Bases827.1 kbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2354.289886820007302No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1442.628696604600219No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.9127418765972983No Hit
ATTGCGCAATGGGGAGGGGGGGAAAAAGAGGAAACCAGTCGGGGAAGAAT370.6754289886820007No Hit
ATTGCGCAATGTGGGCTATGGGGCCCTCCTCTTCCTTTCTCCGCCATCGT340.6206644760861628No Hit
ATTGCGCAATGGTTGCAGGGGGGGGTCAGCGACTTAGTGTATCGAGCAAG320.5841548010222709No Hit
ATTGCGCAATGAAGTACAGGGGCGGATTACCAATCTCAGATAAACTCCGA300.547645125958379No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG300.547645125958379No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG260.4746257758305951No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTC210.3833515881708653No Hit
ATTGCGCAATGGTGCTCGGGGGGGGCGTCAAGGTGAAGCGTCCCGACGAA190.3468419131069734No Hit
ATTGCGCAATGGACCCAAGGGGTCAATATTCCTGTACCGATTGTTAGTGC180.32858707557502737No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA150.2738225629791895No Hit
ATTGCGCAATGGGCTCGGAGGGGGGGCGTCAAGGTGAAGCGTCCCGACGA130.23731288791529756No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG110.2008032128514056No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG100.18254837531945967No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC90.16429353778751368No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.16429353778751368No Hit
ATTGCGCAATGTACGGCAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAG90.16429353778751368No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAT80.14603870025556773No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG80.14603870025556773No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT80.14603870025556773No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC70.12778386272362174No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA70.12778386272362174No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT60.10952902519167579No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA60.10952902519167579No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.10952902519167579No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.10952902519167579No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC60.10952902519167579RNA PCR Primer, Index 40 (96% over 29bp)
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATGG60.10952902519167579No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC60.10952902519167579No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA60.10952902519167579No Hit
ATTGCGCAATGTATGCTGCTGCTGTCTCTTATACACATCTCCGAGCCCAC60.10952902519167579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)