FastQCFastQC Report
Wed 8 May 2024
EGAF00007822282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103608
Total Bases15.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.6399119759091962No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA3770.36387151571307236No Hit
GGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGG3170.30596092965794147No Hit
GAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGG3030.2924484595784109No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT2850.2750752837618717No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCT2730.2634931665508455No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA2510.2422592849972975No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT2260.218129874140993No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG1850.17855764033665353No Hit
GTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAACG1850.17855764033665353No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.17759246390240135No Hit
GTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGAT1770.17083622886263608No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG1760.1698710524283839No Hit
GGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCA1740.16794069955987956No Hit
CTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAG1740.16794069955987956No Hit
GTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAGGT1730.16697552312562736No Hit
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAG1730.16697552312562736No Hit
CTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGG1690.16311481738861866No Hit
GGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCT1470.14188093583507067No Hit
GGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCT1410.13608987722955757No Hit
ATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGC1400.1351247007953054No Hit
GTACTGCGGCGGGTAGGCCTAGGATTGTGGGGGTAATGAATGAAGCGAAC1300.12547293645278357No Hit
ATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCA1280.12354258358427919No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG1250.12064705428152267No Hit
GGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATGAAAG1240.11968187784727048No Hit
TTGTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGC1240.11968187784727048No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG1230.1187167014130183No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.11678634854451393No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC1200.11582117211026176No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA1200.11582117211026176No Hit
GTTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT1200.11582117211026176No Hit
GTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAG1200.11582117211026176No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG1190.11485599567600958No Hit
CGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGG1180.1138908192417574No Hit
GCCTAGGATTGTGGGGGTAATGAATGAAGCGAACAGATTTTCGTTCATTT1170.11292564280750521No Hit
GACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAG1160.11196046637325302No Hit
GCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAG1150.11099528993900085No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG1140.11003011350474866No Hit
GGTATATGGTTAGTGTGTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATG1140.11003011350474866No Hit
AAATAGGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGT1110.10713458420199212No Hit
GAATAGTCCTGTGGTGATTTGGAGGATCAGGCAGGCGCCAAGGAGTGAGC1100.10616940776773995No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA1090.10520423133348776No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC1080.10423905489923557No Hit
GGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAA1080.10423905489923557No Hit
TGCATGGGCTGTGACGATAACGTTGTAGATGTGGTCGTTACCTAGAAGGT1060.10230870203073122No Hit
GGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAG1040.10037834916222686No Hit
GTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA1040.10037834916222686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)