FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT17012.55539143279173No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC493.61890694239291No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGCC231.6986706056129988Illumina Single End PCR Primer 1 (100% over 21bp)
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGG191.4032496307237814No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC181.329394387001477No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGG141.03397341211226No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGCC120.8862629246676514No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGCGCC110.8124076809453471Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTC100.7385524372230428No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.6646971935007385No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGG90.6646971935007385No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGGC80.5908419497784343No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTCGCC70.51698670605613Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGCC70.51698670605613Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.4431314623338257No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGGC60.4431314623338257No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTCGGTGGTCGCC60.4431314623338257Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGGGCC50.3692762186115214No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGGC50.3692762186115214Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGGGGC40.29542097488921715No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGGTGGGC40.29542097488921715No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA40.29542097488921715No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTGGGC40.29542097488921715No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATGTCGG30.22156573116691286No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGG30.22156573116691286No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.22156573116691286No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGG30.22156573116691286No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC30.22156573116691286No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGGGGGCGCC30.22156573116691286No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGGGGGCC30.22156573116691286No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC30.22156573116691286No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATATCGGGGG20.14771048744460857No Hit
GTTTCAGGTACTATTTCACTCCCCTCCCGGGGTACTTTTCACCATTCCCT20.14771048744460857No Hit
CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTACCTCACCAA20.14771048744460857No Hit
GTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCTCGG20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGGGCGGC20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGCGGC20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGGGGTGGCC20.14771048744460857No Hit
ACGTATCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCC20.14771048744460857No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14771048744460857No Hit
GTGTATAAGAGACAGATTGCGTAATCTGTCTCTTATACACATCTGACGCT20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCGCGGTGGTCGGC20.14771048744460857No Hit
GTATAAGAGACAGATTGCGCAATGGGGCCCACGGAAATCATTGCGCAACT20.14771048744460857No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGGGG20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGGTGGCC20.14771048744460857No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGGAGATCTCGGTGGGGGCC20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGCGCC20.14771048744460857No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGGGGTCGCC20.14771048744460857No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC20.14771048744460857No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.14771048744460857No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTAGGTGGTCGCC20.14771048744460857Illumina Single End PCR Primer 1 (95% over 21bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAACTGT20.14771048744460857No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATGTC20.14771048744460857No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATC20.14771048744460857No Hit
TGTCTGTTCTGTGCTATTTTCTTTATTTTTTCTTTTTTTTCAGGTTTCTT20.14771048744460857No Hit
GGCTCATTCTTCAAAAGGCACGCTGTCACCCCACAAGGAGGCTCCAACGG20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTTGTCGCC20.14771048744460857Illumina Single End PCR Primer 1 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCTCCTAGGGTGTAG20.14771048744460857No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAATCTGTGT20.14771048744460857No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATGTG20.14771048744460857No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTC20.14771048744460857No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC20.14771048744460857No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAA20.14771048744460857No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC20.14771048744460857No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGGGGGGC20.14771048744460857No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTGGCC20.14771048744460857No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTAGG20.14771048744460857No Hit
TGTTTATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG20.14771048744460857No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.14771048744460857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)