FastQCFastQC Report
Tue 7 May 2024
EGAF00007822296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4354
Total Bases657.4 kbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3036.959118052365641No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1663.812586127698668No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA801.8373909049150206No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGG571.3091410197519522No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441.0105649977032614No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTC430.9875976113918236No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGG270.6201194304088194No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.5971520440973818No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.5282498851630685No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC210.482315112540193No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGCC190.4363803399173174Illumina Single End PCR Primer 1 (100% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG170.39044556729444185No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG160.3674781809830041No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGG160.3674781809830041No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.2985760220486909No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG110.25264124942581534No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG110.25264124942581534No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGCC110.25264124942581534Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA110.25264124942581534No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGGC110.25264124942581534Illumina Single End PCR Primer 1 (95% over 21bp)
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTG100.22967386311437757No Hit
GTTTAGCCCCATCCGCGTTCGCTCGCCACTACTAACGGAATCACTTTTGT90.20670647680293983No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC90.20670647680293983No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCT80.18373909049150206No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC80.18373909049150206No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG80.18373909049150206No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGCGCC80.18373909049150206Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGCGCC80.18373909049150206No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTCGG80.18373909049150206No Hit
CATACGGTTTAGCCCCATCCGCGTTCGCTCGCCACTACTAACGGAATCAC80.18373909049150206No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGGGGC70.1607717041800643No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGGC70.1607717041800643No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTCGGTGGTCGCC70.1607717041800643Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATATC70.1607717041800643No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC70.1607717041800643No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATGTCGG60.13780431786862654No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA60.13780431786862654No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC60.13780431786862654No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.13780431786862654No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13780431786862654No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATATCGG60.13780431786862654No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.13780431786862654No Hit
CTATTTCACTCCCCTCCCGGGGTACTTTTCACCATTCCCTCACGGTACTG60.13780431786862654No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC60.13780431786862654No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC60.13780431786862654No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT50.11483693155718878No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGG50.11483693155718878No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA50.11483693155718878No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG50.11483693155718878No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGGGCC50.11483693155718878No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGCC50.11483693155718878No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGG50.11483693155718878No Hit
GCCCACAACACCGCACATGCAACCCCTGCCGGGTATCACACACATACGGT50.11483693155718878No Hit
GTCTATGTGTTCAACCGGGGGTCACACGACACGTCGTGCGGGGTTTCCCC50.11483693155718878No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)