FastQCFastQC Report
Tue 7 May 2024
EGAF00007822297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14654
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG4973.3915654428824897No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4142.825167189845776No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT3772.572676402347482No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2771.8902688685683091No Hit
ATTGCGCAATGATTTGCAAGGGGGAAGGGCGGGGAACGCGCTAAGTGTTG2221.5149447249897638No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.6346390064146309No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG890.6073427050634639No Hit
ATTGCGCAATGAGTGGTCAGGGTAGTACAAATTACGGAAAGAGCCAGCGA850.580046403712297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC760.5186297256721715No Hit
ATTGCGCAATGTGCAGCAGGGGCGCAATGGTGTTGGGAGAACTACTTGTC710.4845093489832128No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAT690.4708611983076293No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT620.4230926709430872No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT580.39579636959192027No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTC570.38897229425412855No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT500.3412037668895865No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG460.3139074655384196No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG370.25249078749829396No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT340.23201856148491878No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG340.23201856148491878No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC330.22519448614712706No Hit
ATTGCGCAATGGCGTAGTGGGGAGGCCGACGAGGAGCGCCTTGGCAAGCT320.21837041080933536No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG270.1842500341203767No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA270.1842500341203767No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.17742595878258496No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGC260.17742595878258496No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT260.17742595878258496No Hit
ATTGCGCAATGTATCCGCGGGGGGGCAGTGGCTTTGAGAACAAAGACAGT250.17060188344479324No Hit
ATTGCGCAATGAGGGGGGCGGGTCACTCGTTGTGTTGAGTATTGATGTGT240.1637778081070015No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC240.1637778081070015No Hit
ATTGCGCAATGTCTCGCGTGGGGTTTGCGGCCGAGATTGCGCACAACGTG240.1637778081070015No Hit
ATTGCGCAATGGAGTCCGAGGGGCAAGGTGATCGACGGCGCCGAGTATGG240.1637778081070015No Hit
ATTGCGCAATGTGTGGAACGGGCGGGTAACGGGGAATAAGGGTTCGATTC240.1637778081070015No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240.1637778081070015No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA220.15012965743141804No Hit
ATTGCGCAATGTGTGGAGAGGGAATGTGAAGTTTTGAAGTCATTTCTTTT220.15012965743141804No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG220.15012965743141804No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT210.14330558209362632No Hit
ATTGCGCAATGACGCCAGTGGGGATGACTCACCCGTCTTGGCGTCTTTGC210.14330558209362632No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.13648150675583456No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCGCG200.13648150675583456No Hit
ATTGCGCAATGGTCTTGGTGGGGCTCATTCTCTATGTCCAAAATATTTGA190.12965743141804287No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.12965743141804287No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA180.12283335608025113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.12283335608025113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.11600928074245939No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.11600928074245939No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT170.11600928074245939No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.10918520540466768No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATGG160.10918520540466768No Hit
ATTGCGCAATGATGTGTAGGGGGAGTTTGGCTGGGGCGGCACATCTGTAA160.10918520540466768No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150.10236113006687594No Hit
ATTGCGCAATGGATAACATGGGGAAATCGCTTTCCCATGCTCCGCTATCG150.10236113006687594No Hit
ATTGCGCAATGCGCTAGCCGGGCAACTAAGCGACTCGTATGTGTAAGGTT150.10236113006687594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC150.10236113006687594No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCG150.10236113006687594No Hit
ATTGCGCAATGAACCTCGAGGGCAGACCGCCAGCTAAGGTCCCTAAAATT150.10236113006687594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)