FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14654
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTA5904.02620444929712No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5273.596287703016241No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3592.449843046267231No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGA1541.0509076020199264No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTT1410.9621946226286338No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGG680.46403712296983757No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTG670.45721304763204584No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.4162685956052955No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG590.40262044492971205No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC580.39579636959192027No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGT560.3821482189163368No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC540.3685000682407534No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.3685000682407534No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA450.3070833902006278No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAG430.29343523952504436No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG360.24566671216050226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG350.23884263682271054No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.21837041080933536No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC290.19789818479596014No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA280.1910741094581684No Hit
GTGCTCCGCGCCTTCAAGATGAAGTTGTGCATGAGGTTGGCATACTGTGT260.17742595878258496No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC250.17060188344479324No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.1637778081070015No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGG230.1569537327692098No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCA230.1569537327692098No Hit
CTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTCGG210.14330558209362632No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT200.13648150675583456No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT190.12965743141804287No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.12965743141804287No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC190.12965743141804287No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA170.11600928074245939No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA170.11600928074245939No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTC170.11600928074245939No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11600928074245939No Hit
CATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTCGGTGGTCGCC160.10918520540466768Illumina Single End PCR Primer 1 (100% over 21bp)
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAG160.10918520540466768No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA150.10236113006687594No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA150.10236113006687594No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA150.10236113006687594No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC150.10236113006687594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)