FastQCFastQC Report
Tue 7 May 2024
EGAF00007822299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7301
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3354.588412546226545No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1972.698260512258595No Hit
ATTGCGCAATGGATTCCGAGGGGGAAAAATAAAGAGAGAAAAGAGAAAAC1031.4107656485412958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA781.0683468018079714No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG440.6026571702506506No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC390.5341734009039857No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTC370.5067798931653198No Hit
ATTGCGCAATGGAAACCCGGGGTCAAGTGAAGAAGGGCGCACGGTGAATG360.49308313929598685No Hit
ATTGCGCAATGTCCGGTCGGGGGGAAAAATAAAGAGAGAAAAGAGAAAAC360.49308313929598685No Hit
ATTGCGCAATGTCAGGACGGGGGGAAAAATAAAGAGAGAAAAGAGAAAAC290.397205862210656No Hit
ATTGCGCAATGATTCGTAGGGGAAGAGAAAGAAAAGAATACGGAATGATA260.3561156006026572No Hit
ATTGCGCAATGAAGTCCAGGGGGAGGCACCCACACCCGCTGAGGGAACGC250.3424188467333242No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG240.32872209286399123No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG230.3150253389946583No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA230.3150253389946583No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA210.28763183125599234No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT200.27393507738665934No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAT170.23284481577866045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC140.19175455417066153No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG120.16436104643199562No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA100.13696753869332967No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.13696753869332967No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG100.13696753869332967No Hit
ATTGCGCAATGGACGAGTCGGGGGATAAGCTTAACATAGTAGGTATTTAC100.13696753869332967No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC80.10957403095466374RNA PCR Primer, Index 40 (96% over 29bp)
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA80.10957403095466374No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA80.10957403095466374No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT80.10957403095466374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)