FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7301
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4305.889604163813177No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA3544.848650869743871No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2493.410491713463909No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG1461.9997260649226134No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC991.3559786330639638No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT951.301191617586632No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC841.1505273250239694Illumina Single End PCR Primer 1 (100% over 22bp)
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCA620.849198739898644No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.794411724421312No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG490.6711409395973155No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG400.5478701547733187No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC370.5067798931653198No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.49308313929598685No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTG310.424599369949322No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC300.41090261607998907No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC280.38350910834132307Illumina Single End PCR Primer 1 (95% over 22bp)
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG280.38350910834132307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC230.3150253389946583No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.27393507738665934No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.2602383235173264No Hit
ATTTTATACTTCTCTTTCTGTTCATTTTTTTTATTTTCTTGTTTCACTTT180.24654156964799342No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCC170.23284481577866045Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG160.21914806190932748No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG150.20545130803999453No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC140.19175455417066153No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.1780578003013286No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTC130.1780578003013286No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG130.1780578003013286No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC130.1780578003013286No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG120.16436104643199562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.16436104643199562No Hit
CCTTTACTCTCCATCCCTTTTTTCTTTCTACCTCATTTTTTATACTCTTC120.16436104643199562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG120.16436104643199562No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC110.15066429256266264No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA110.15066429256266264No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTA110.15066429256266264No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC110.15066429256266264Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGGC100.13696753869332967No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCC100.13696753869332967Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA100.13696753869332967No Hit
CTCTTCTTCTTTTCCCATTTATTTTATACTTCTCTTTCTGTTCATTTTTT90.12327078482399671No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGG90.12327078482399671No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.12327078482399671No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA80.10957403095466374No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT80.10957403095466374No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA80.10957403095466374No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTC80.10957403095466374No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT80.10957403095466374No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT80.10957403095466374No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA80.10957403095466374No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA80.10957403095466374No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA80.10957403095466374No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGGGCC80.10957403095466374No Hit
CTTTTACTACGTCATTTCTTTTTAACTTCCATTTGCTCTATTATTTTTTT80.10957403095466374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)