FastQCFastQC Report
Tue 7 May 2024
EGAF00007822301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17919
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC7944.431050839890619No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC5132.862882973380211No Hit
ATTGCGCAATGGACCACCCGGGAGAGAAGCAAGGCCAGCACAGAGTCTGC4242.366203471175847No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT3602.0090406830738323No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG3581.9978793459456443No Hit
ATTGCGCAATGTGAGGGGCGGGCCGGCGAAGGCCGGTGCGTCGGATCAGA2251.255650426921145No Hit
ATTGCGCAATGTGAGGGGTGGGCCGGCGAAGGCCGGTGCGTCGGATCAGA2011.1217143813828898No Hit
ATTGCGCAATGCAAATGCGGGGGAAGGTTGAGGCATGATGGGGAGCCCAT1740.9710363301523522No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.9542943244600702No Hit
ATTGCGCAATGCCAGGGCTGGGTGTGGTGGCTCACACCTGTAATTCCCAG1540.8594229588704727No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1030.5748088621016798No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATA1020.5692281935375858No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG970.5413248507171159No Hit
ATTGCGCAATGTTGCCGCGGGGAGTGGTAATTGGGTCGTTTGCTAGCGTG940.524582845024834No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCG890.4966795022043641No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT770.42971147943523635No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT770.42971147943523635No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATAT710.3962274680506725No Hit
ATTGCGCAATGGCCCGCGCGGGAGTGGTAATTGGGTCGTTTGCTAGCGTG700.3906467994865785No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCT570.3180981081533568No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG550.3069367710251688No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC530.29577543389698085No Hit
ATTGCGCAATGATTCCATAGGGAGGACAGTGGATTTGGGAAGTGTTTGCA520.29019476533288685No Hit
ATTGCGCAATGAGTACTGGGGGGGGCGCCGGCGGCTTTGGTGACTCTAGA500.27903342820469895No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACA490.27345275964060495No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT480.26787209107651094No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC480.26787209107651094No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT440.24554941682013504No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT430.23996874825604106No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG410.22880741112785313No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG390.21764607399966515No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC370.20648473687147723No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG350.19532339974328924No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC340.18974273117919527No Hit
ATTGCGCAATGGTTGGGGTGGGCCGGCGAAGGCCGGTGCGTCGGATCAGA340.18974273117919527No Hit
ATTGCGCAATGGCGCTTAGGGGGGACTAACACCGATATGCTTCGGGGAGC320.17858139405100731No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA320.17858139405100731No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG320.17858139405100731No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.16742005692281936No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC300.16742005692281936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG290.16183938835872538No Hit
ATTGCGCAATGATAACGGCGGGAGCGGTGTTCCGGCCGTTGTCGCTGGTG280.15625871979463138No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA270.15067805123053743No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA260.14509738266644343No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC260.14509738266644343No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT250.13951671410234948No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.13393604553825547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG230.1283553769741615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG210.11719403984597354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT200.11161337128187958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT200.11161337128187958No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC200.11161337128187958No Hit
ATTGCGCAATGAATCGCCCGGGTGGTGGATAGGTAGAGTATACCGAGGCG200.11161337128187958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT200.11161337128187958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.10603270271778559No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT190.10603270271778559No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.10603270271778559No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCCTG180.10045203415369162No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG180.10045203415369162No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT180.10045203415369162No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC180.10045203415369162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)