FastQCFastQC Report
Tue 7 May 2024
EGAF00007822302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17919
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT10185.68112059824767No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC6503.627434566661086No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTA5813.2423684357386016No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTT1500.8371002846140967No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGA1500.8371002846140967No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGG1150.6417768848708075No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.5580668564093979No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTG850.4743568279479882No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC800.4464534851275183No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.3627434566661086No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGT580.32367877671745077No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA520.29019476533288685No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAG480.26787209107651094No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG470.262291422512417No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC420.23438807969194708No Hit
GTCCAGGTTCAACCGGATGCACTGCCTCAGGTCGGCGACCATGTCCTCGG410.22880741112785313No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC400.22322674256375916No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG400.22322674256375916No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC330.1841620626151013No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG310.17300072548691334No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.16183938835872538No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGG290.16183938835872538No Hit
CCCCTGGACTCTGCGCCCCTCACCGCCACCACACCGCACGGGTGCACGAC290.16183938835872538No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.15067805123053743No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT260.14509738266644343No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC240.13393604553825547No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.13393604553825547No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG230.1283553769741615No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAG230.1283553769741615No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA220.12277470841006752No Hit
GCTATAGCCTCCTCGACCCACTTCTGATGGGCTTCAGCGCAGACTTCCAT220.12277470841006752No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA220.12277470841006752No Hit
CTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTCGG220.12277470841006752No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.12277470841006752No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT210.11719403984597354No Hit
GCCTCAGGTCGGCGACCATGTCCTCGGCCAGGCAGGCGTCCGCGCAGGCG210.11719403984597354No Hit
CTTCCATGCCCTTCTTTGTCTTGAAGATAACAGCTTCCCAGGGACACCGA210.11719403984597354No Hit
CTATAATACAAGTATTTTCTACATTCAAGTAGGAGTAAGACGGAAGAGAG200.11161337128187958No Hit
GACTACTGGTGATCCTTCTGTAGCTCTTGAGATTCCTCTTGGGGATCTTT200.11161337128187958No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA190.10603270271778559No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA190.10603270271778559No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC190.10603270271778559No Hit
CCACCACACCGCACGGGTGCACGACAGCTCAGGTCCCCCTTATCCCAAAG180.10045203415369162No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA180.10045203415369162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)