FastQCFastQC Report
Tue 7 May 2024
EGAF00007822305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813
Total Bases273.7 kbp
Sequences flagged as poor quality0
Sequence length151
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC19410.70049641478213No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC804.4125758411472695No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG150.827357970215113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.7722007722007722No Hit
ATTGCGCAATGAGGGTGGGGGGGGGGCTAAGGGAGCAATGCCAAGCTTCA90.4964147821290678No Hit
ATTGCGCAATGAGAGCGGGGGGGGGGCTAAGGGAGCAATGCCAAGCTTCA80.441257584114727No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.3861003861003861No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC50.27578599007170435No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCT40.2206287920573635No Hit
ATTGAGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC40.2206287920573635No Hit
GGCTTAGGGCCGGGGGGGGCAAGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.2206287920573635No Hit
ATTGCGCAATCTGTCTCTTATACACCGGTCTCTTATACACATCTCCGAGC40.2206287920573635No Hit
GGGGGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.2206287920573635No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.2206287920573635No Hit
ATTGCGCAATATGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC40.2206287920573635No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1654715940430226No Hit
ATTGCGCAATCTGGCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC30.1654715940430226No Hit
ATTGCGCAATATGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC30.1654715940430226No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCATATACACATCTCCGAGC30.1654715940430226No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACAAATCTCCGAGC30.1654715940430226No Hit
ATTGCGCAATCTGTCTCTTATACCTGGCTCTTATACACATCTCCGAGCCC30.1654715940430226No Hit
GCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1654715940430226No Hit
GCGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1654715940430226No Hit
ATTGCGCAATCTGTCTCTTATACACACTGTCTCTTATACACATCTCCGAG20.11031439602868175No Hit
ATTGCACAATGAGAGCGGGGGGGGGGCTAAGGGAGCAATGCCAAGCTTCA20.11031439602868175No Hit
GGGTGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
ATTGCGCAATCTGTCTCTAATACACCTGTCTCTTATACACATCTCCGAGC20.11031439602868175No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTATCTTATACACATCTCCGAGC20.11031439602868175No Hit
GGGCGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
GCGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
ATTGCGCAATGAGAGCGGGGGGGGGGCTAAGGGAGCAATGCCAATCTTCA20.11031439602868175No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC20.11031439602868175RNA PCR Primer, Index 40 (96% over 29bp)
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG20.11031439602868175No Hit
GGCGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
ATTGCGCAATCTGTATCTTATACACCTGTCTCTTATACACATCTCCGAGC20.11031439602868175No Hit
ATTGCGCAATGTGCCCGCCGGGAACATTGCCAATCTGTCTCTTATACACC20.11031439602868175No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTTTTGGGGGGG20.11031439602868175No Hit
GGGTGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCGCTTATACACATCTCCG20.11031439602868175No Hit
GTGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGATGCCT20.11031439602868175No Hit
ATTGCGCAATGAGAGCGGGGGGGGGGCTAAGGGAGCAATGCCAAGCATCA20.11031439602868175No Hit
ATTGCGCAATGGCCAACGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAA20.11031439602868175No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.11031439602868175No Hit
GGGCGCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
ATTGCGCAATGAGAGCGGGGGGGGGCTAAGGGAGCAATGCCAAGCTTCAG20.11031439602868175No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTAAACACATCTCCGAGC20.11031439602868175No Hit
GCCTTGTGGCAGGGGGGGGCAGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
GGGGGAGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11031439602868175No Hit
ATTGCGCAATGAGTGGGGGGGGGGCTAAGGGAGCAATGCCAAGCTTCAGT20.11031439602868175No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT20.11031439602868175No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTGTTATACACATCTCCGAGC20.11031439602868175No Hit
ATTGCGCAATCTGTCTCTTATACACGTGTCTCTTATACACATCTCCGAGC20.11031439602868175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)