FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT27815.33370104798676No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1196.563706563706563No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC774.247104247104247Illumina Single End PCR Primer 1 (100% over 22bp)
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG623.419746276889134No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG331.8201875344732488No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC301.654715940430226No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC291.5995587424158852No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA281.5444015444015444No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241.323772752344181No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC191.0479867622724766Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.827357970215113No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC140.7722007722007722Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGGC110.6067291781577496No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCC110.6067291781577496Illumina Single End PCR Primer 1 (95% over 22bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.5515719801434087No Hit
GCCTACACCTTCAGTGGTACATGTGCAATGCCAATATAGGCTATTTGAGG90.4964147821290678No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCC90.4964147821290678Illumina Single End PCR Primer 1 (95% over 22bp)
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG80.441257584114727No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGG60.3309431880860452No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.3309431880860452No Hit
CACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCG60.3309431880860452No Hit
GTGTATAAGAGACAGAGTGCGCAATCTGTCTCTTATACACATCTGACGCT60.3309431880860452No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCG50.27578599007170435Illumina Single End PCR Primer 1 (95% over 21bp)
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGG50.27578599007170435No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG40.2206287920573635No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC40.2206287920573635No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2206287920573635No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC40.2206287920573635No Hit
TCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCCGG40.2206287920573635Illumina Single End PCR Primer 1 (95% over 23bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGGCGGC30.1654715940430226No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGTCGGC30.1654715940430226No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1654715940430226No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACAGGAAGTGCCAGTGTAG30.1654715940430226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA30.1654715940430226No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCG30.1654715940430226Illumina Single End PCR Primer 1 (95% over 21bp)
GCCAAGCATGAGACCTGCAGTGGGTTTAACCACATCAGGGCAAGCAAAGG30.1654715940430226No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCA30.1654715940430226No Hit
TGCCAATATAGGCTATTTGAGGTTTCAGAACTTCACAAGTAGATTCAGAT30.1654715940430226No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTT30.1654715940430226No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGGTCTCGG30.1654715940430226No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCGCGGTGGGCGCC30.1654715940430226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC30.1654715940430226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC30.1654715940430226No Hit
CATGAGACCTGCAGTGGGTTTAACCACATCAGGGCAAGCAAAGGGCTCCA30.1654715940430226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC30.1654715940430226No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTC20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGGCGCC20.11031439602868175No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAATA20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGTCGCC20.11031439602868175Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTAT20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGGG20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTATATATCGGTGGTCGCC20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGGCGCC20.11031439602868175No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTTAG20.11031439602868175No Hit
NTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT20.11031439602868175No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAAA20.11031439602868175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.11031439602868175No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGGGG20.11031439602868175No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACCATCTGA20.11031439602868175No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG20.11031439602868175No Hit
GCATGAGACCTGCAGTGGGTTTAACCACATCAGGGCAAGCAAAGGGCTCC20.11031439602868175No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAAAAATT20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGGGGG20.11031439602868175No Hit
GGTTTAACCACATCAGGGCAAGCAAAGGGCTCCATCTGTCTTCACTGGGG20.11031439602868175No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA20.11031439602868175No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAACT20.11031439602868175No Hit
TATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTG20.11031439602868175No Hit
GAAGAGCACACGTCTGAACTCCAGTCACAGTCAGAATCTCGTATGCCGTC20.11031439602868175TruSeq Adapter, Index 13 (96% over 33bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGG20.11031439602868175No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATT20.11031439602868175No Hit
AGTAGATTCAGATCTCATTGGACAGCCAAGCATGAGACCTGCAGTGGGTT20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGACGTGCCAGTGTAGATCTCGGTGGTCGCC20.11031439602868175Illumina Single End PCR Primer 1 (100% over 22bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGGG20.11031439602868175No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG20.11031439602868175No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGGTCTCGGTGG20.11031439602868175No Hit
CCTTCAGTGGTACATGTGCAATGCCAATATAGGCTATTTGAGGTTTCAGA20.11031439602868175No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCGCGG20.11031439602868175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA20.11031439602868175No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11031439602868175No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11031439602868175No Hit
TTATTGGACAGCCTACACCTTCAGTGGTACATGTGCAATGCCAATATAGG20.11031439602868175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCCAA20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGGTCTCGGTGGTCGCC20.11031439602868175Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGAGCTCGGTGGGCGCC20.11031439602868175No Hit
GAGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT20.11031439602868175No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG20.11031439602868175No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA20.11031439602868175No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCGCGGTGG20.11031439602868175No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAACTTTG20.11031439602868175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCC20.11031439602868175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC20.11031439602868175No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA20.11031439602868175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG20.11031439602868175No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATGTCGGTGG20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCGCGGTGGGCGGC20.11031439602868175No Hit
ATTTGAGGTTTCAGAACTTCACAAGTAGATTCAGATCTCATTGGACAGCC20.11031439602868175No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATATCGGTGGTCGCC20.11031439602868175Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.11031439602868175No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAT20.11031439602868175No Hit
GTGTATAAGAGACAGATTGGCAATGTTCCCGGCGGGCACATTGCGCAATC20.11031439602868175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)