FastQCFastQC Report
Tue 7 May 2024
EGAF00007822307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5277
Total Bases796.8 kbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2354.45328785294675No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1162.1982186848588214No Hit
ATTGCGCAATGCTAATACCGGGGGGGTGGGTGAGTGTGTGAACCGATCAT701.3265112753458403No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA531.004358537047565No Hit
ATTGCGCAATGTTTTGGGTGGGGATGAAAGACGTGGTAGCCTGCGATAAG310.5874549933674436No Hit
ATTGCGCAATGGCAGCGAGGGGGAAGGTTGAGGCATGATGGGGAGCCCAC290.5495546712147054No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.37900322152738297No Hit
ATTGCGCAATGGGGTAGGTGGGAAAGGAAAGAAGGCTGCTGTTGGTGTTA160.30320257722190636No Hit
ATTGCGCAATGTGTGGGGAGGGGTGTTCCAAAGAGAAGGCCTGGTCAGCA160.30320257722190636No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACAT150.28425241614553726No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG150.28425241614553726No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAG150.28425241614553726No Hit
ATTGCGCAATGACTTACTGGGGGGGAAAAAAAAAAACACAAAAAGGGAGA130.24635209399279895No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC130.24635209399279895No Hit
ATTGCGCAATGGCATATTGGGGAAGTTGATTTGAGATTAGTGGAACGCTC100.18950161076369149No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.17055144968732233No Hit
ATTGCGCAATGTAAGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA90.17055144968732233No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA80.15160128861095318No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCATCG80.15160128861095318No Hit
ATTGCGCAATGCGGTGGCCGGGGCAGCCCTTCAGGAGCTCCTTAGTAAAG80.15160128861095318No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGGACATCAT70.13265112753458402No Hit
ATTGCGCATGATTATAACGGGGGGAAAAAAAAAAACACAAAAAGGGAGAG70.13265112753458402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT60.11370096645821488No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC60.11370096645821488RNA PCR Primer, Index 40 (96% over 29bp)
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG60.11370096645821488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)