FastQCFastQC Report
Tue 7 May 2024
EGAF00007822308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5277
Total Bases796.8 kbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2975.628197839681637No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1502.8425241614553722No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA1382.6151222285389424No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG1212.292969490240667No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC901.7055144968732234No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC811.534963047185901Illumina Single End PCR Primer 1 (100% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT611.1559598256585182No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.8338070873602426No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG410.7769566041311351No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC290.5495546712147054Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG270.511654349061967No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.511654349061967No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCA260.4927041879855979No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG220.4169035436801213No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC210.3979533826037522No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG180.34110289937464466No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.26530225506916805No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTG140.26530225506916805No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG140.26530225506916805No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.26530225506916805No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA130.24635209399279895No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC130.24635209399279895No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC120.22740193291642977Illumina Single End PCR Primer 1 (95% over 22bp)
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC110.20845177184006064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG110.20845177184006064No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCC110.20845177184006064Illumina Single End PCR Primer 1 (95% over 22bp)
GGTATACTCTACCTATCCACCAGTGTCGGTTTAGGGTACGGGCGGACCCG110.20845177184006064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC110.20845177184006064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC100.18950161076369149No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTC100.18950161076369149No Hit
CCACCACACCGCACGGGTGCACGACAGCTCAGGTCCCCCTTATCCCAAAG100.18950161076369149No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG90.17055144968732233No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCG90.17055144968732233Illumina Single End PCR Primer 1 (95% over 21bp)
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCC80.15160128861095318No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGGC80.15160128861095318No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCC80.15160128861095318Illumina Single End PCR Primer 1 (95% over 22bp)
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCGCGG80.15160128861095318No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC80.15160128861095318No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA80.15160128861095318No Hit
CCCCTGGACTCTGCGCCCCTCACCGCCACCACACCGCACGGGTGCACGAC80.15160128861095318No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA70.13265112753458402No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA70.13265112753458402No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA70.13265112753458402No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA70.13265112753458402No Hit
GTATACTCTACCTATCCACCAGTGTCGGTTTAGGGTACGGGCGGACCCGC70.13265112753458402No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAACT70.13265112753458402No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA70.13265112753458402No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAA70.13265112753458402No Hit
GTACAGGATCACTCACTCACCCACAACCGTGGGCTCCCCATCATGCCTCA70.13265112753458402No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.13265112753458402No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC70.13265112753458402No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT60.11370096645821488No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA60.11370096645821488No Hit
CATCAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTG60.11370096645821488No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.11370096645821488No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGT60.11370096645821488No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA60.11370096645821488No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA60.11370096645821488No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG60.11370096645821488No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC60.11370096645821488No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA60.11370096645821488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)