FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5260
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA2715.152091254752852No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1051.9961977186311788No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC801.520912547528517Illumina Single End PCR Primer 1 (100% over 22bp)
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG741.4068441064638784No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC721.3688212927756656No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC541.0266159695817492No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.8745247148288974No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG400.7604562737642585No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC310.5893536121673004No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.5893536121673004No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCA300.5703422053231939No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG260.494296577946768No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG250.4752851711026616No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA250.4752851711026616No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTG240.45627376425855515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG240.45627376425855515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.4372623574144487No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC190.3612167300380228Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG170.3231939163498099No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC170.3231939163498099No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC170.3231939163498099Illumina Single End PCR Primer 1 (95% over 22bp)
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG160.3041825095057034No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.2661596958174905No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA130.247148288973384No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC120.22813688212927757No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC120.22813688212927757No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC120.22813688212927757No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.22813688212927757No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGA100.19011406844106463No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCC100.19011406844106463Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGT100.19011406844106463No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.19011406844106463No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC100.19011406844106463No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATGT90.1711026615969582No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA90.1711026615969582No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA90.1711026615969582No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA80.1520912547528517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA80.1520912547528517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG80.1520912547528517No Hit
TCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA70.13307984790874525No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT70.13307984790874525No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA70.13307984790874525No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA70.13307984790874525No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC70.13307984790874525No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCG70.13307984790874525Illumina Single End PCR Primer 1 (95% over 21bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.13307984790874525No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA70.13307984790874525No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA60.11406844106463879No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATTTA60.11406844106463879No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAAT60.11406844106463879No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCC60.11406844106463879Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGGC60.11406844106463879No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA60.11406844106463879No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA60.11406844106463879No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA60.11406844106463879No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG60.11406844106463879No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGTCGCC60.11406844106463879Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTC60.11406844106463879No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCCA60.11406844106463879No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC60.11406844106463879No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT60.11406844106463879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)