FastQCFastQC Report
Tue 7 May 2024
EGAF00007822314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6698
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4937.3604060913705585No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2774.1355628545834575No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA2243.344281875186623No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG1241.8512988951925948No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC771.1495968945954016No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC761.1346670647954613Illumina Single End PCR Primer 1 (100% over 22bp)
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG570.8510002985965961No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.7464914899970141No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC480.7166318303971335No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.4926843833980293No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC280.41803523439832785Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG270.40310540459838756No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC260.38817557479844733Illumina Single End PCR Primer 1 (95% over 22bp)
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCA230.3433860853986265No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG220.32845625559868613No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.32845625559868613No Hit
TATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACATCCA210.3135264257987459No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG190.2836667661988653No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTG180.26873693639892504No Hit
GCCTATTAGTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACAT170.25380710659898476No Hit
GTTCTAGCTGGGGGCCTCACACCCTCAAAGGGGCAAGGAAATCTCATCTC170.25380710659898476No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG160.2388772767990445No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.2388772767990445No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCG160.2388772767990445No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC150.2239474469991042No Hit
CCCTCAAAGGGGCAAGGAAATCTCATCTCGAAGCCGGCTTCCCGCTTAGA150.2239474469991042No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA150.2239474469991042No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC150.2239474469991042No Hit
TTATAAGAGTGTGTGTAAGTCATCGGCCTATTAGTACTGGTCAGCTTCAC150.2239474469991042No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA140.20901761719916392No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA130.19408778739922367No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG130.19408778739922367No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.19408778739922367No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.17915795759928338No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC110.16422812779934307No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCC110.16422812779934307Illumina Single End PCR Primer 1 (95% over 22bp)
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG110.16422812779934307No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTC110.16422812779934307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA110.16422812779934307No Hit
GTGTAAGTCATCGGCCTATTAGTACTGGTCAGCTTCACGAGTCTTCAGTC110.16422812779934307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA100.1492982979994028No Hit
GTGTGTAAGTCATCGGCCTATTAGTACTGGTCAGCTTCACGAGTCTTCAG100.1492982979994028No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC100.1492982979994028No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGGC90.13436846819946252No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGGC90.13436846819946252No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG90.13436846819946252No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTTA90.13436846819946252No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC80.11943863839952225No Hit
GTATAAGAGACAGAGTGCGCAATCTGTCTCTTATACACATCTGACGCTGC80.11943863839952225No Hit
ACATATAACTTTGCATTTCTTACCCTGCTACAGCATCGTCTATAAAGACA80.11943863839952225No Hit
GTGCACACACAGGACACCCTTCTAGCATGAAGAACTTTTGCACCGTGTCC80.11943863839952225No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATGT80.11943863839952225No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT70.10450880859958196No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGA70.10450880859958196No Hit
GTACTGGTCAGCTTCACGAGTCTTCAGTCCTCGCTTCCACATCCAGCCTA70.10450880859958196No Hit
GAGTTAGCCGGAGCTTATTCTCCCGGTACTGTCATTATCATCCCGGGTAA70.10450880859958196No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCC70.10450880859958196Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC70.10450880859958196No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCG70.10450880859958196Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGGGCC70.10450880859958196No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA70.10450880859958196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)