FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5785
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3245.600691443388072No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1492.575626620570441No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA621.0717372515125323No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG320.5531547104580812No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGAT190.32843560933448573No Hit
ATTGCGCAATGATCGGGGGGGGGATGAAAGAGACAGCAGCTCCTGGTCAA160.2765773552290406No Hit
ATTGCGCAATGGTGTTGTGGGCCCCTCCTTGTCATGTCTGCCGGCATGGC150.25929127052722556No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.24200518582541053No Hit
ATTGCGCAATGCGATTTCAGGGAAGTTTCCAAGGGCGCATGGTGGATGCC120.20743301642178047No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA120.20743301642178047No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG110.19014693171996544No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA110.19014693171996544No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.17286084701815038No Hit
ATTGCGCAATGAGGGAGGGGGGTTCTCCTTTGCCCTCAGCTTTCCCCAGC90.15557476231633535No Hit
ATTGCGCAATGGATTAATGGGGGGAGGTCTAAACCAAAACACACAAAAAA90.15557476231633535No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG90.15557476231633535No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA80.1382886776145203No Hit
ATTGCGCAATGGGAGGGGCGGGCATATAGACGAACCAGTGTGGATGCTGG80.1382886776145203No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG80.1382886776145203No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG70.12100259291270526No Hit
ATTGCGCAATGTTCAGTTCGGGGGAGCTGAAGGAGTTGATCCAGAAGGAG70.12100259291270526No Hit
ATTGCGCAATGGGGGGGTATGGCGCAGTTGGTAGCGCGTCTGCATGGCAT70.12100259291270526No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.12100259291270526No Hit
ATTGCGCAATGGTCTCTAGGGGGGGGGTATGGCGCAGTTGGTAGCGCGTC70.12100259291270526No Hit
ATTGCGCAATGATATGGCCGGGCGGCCGGGTCCGGTCCGCACCGACCGGC60.10371650821089023No Hit
ATTGCGCAATGTGGCGAGTGGGGGAGCGGTTCGCGGGCCAGGTGCGCAGC60.10371650821089023No Hit
ATTGCGCAATGAAGGACGTGGGAATCTGCGATAAGCCTGGTGGAGTCGAT60.10371650821089023No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC60.10371650821089023RNA PCR Primer, Index 40 (96% over 29bp)
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAT60.10371650821089023No Hit
ATTGCGCAATGGGTGCCGCGGGGTGAGAAAATTAAAAAAAAAAAATCAAC60.10371650821089023No Hit
ATTGCGCAATGTCGAGCTTGGGGGATGGTAGAGTATACCAAGGCGCTTGA60.10371650821089023No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC60.10371650821089023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)