FastQCFastQC Report
Tue 7 May 2024
EGAF00007822316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5785
Total Bases873.5 kbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3996.8971477960242No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2093.6127917026793432No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA2023.4917891097666383No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG1382.3854796888504755No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC861.4866032843560935Illumina Single End PCR Primer 1 (100% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC701.2100259291270528No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT691.1927398444252377No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG420.7260155574762317No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC410.7087294727744167No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.6914433880726015No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCA370.6395851339671564No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.44943820224719105No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCC210.36300777873811585Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC210.36300777873811585Illumina Single End PCR Primer 1 (95% over 22bp)
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTG200.34572169403630076No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA190.32843560933448573No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG190.32843560933448573No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCC180.3111495246326707Illumina Single End PCR Primer 1 (95% over 22bp)
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG180.3111495246326707No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG170.2938634399308557No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG170.2938634399308557No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC160.2765773552290406No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.2765773552290406No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.25929127052722556No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC150.25929127052722556No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC140.24200518582541053No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG140.24200518582541053No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG120.20743301642178047No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC120.20743301642178047No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA110.19014693171996544No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG110.19014693171996544No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC110.19014693171996544Illumina Single End PCR Primer 1 (95% over 22bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT100.17286084701815038No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.17286084701815038No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCC90.15557476231633535No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA90.15557476231633535No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC90.15557476231633535No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA90.15557476231633535No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTC80.1382886776145203No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA80.1382886776145203No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTTAT80.1382886776145203No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATTT80.1382886776145203No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT70.12100259291270526No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT70.12100259291270526No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGA70.12100259291270526No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC70.12100259291270526No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGGC70.12100259291270526No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT70.12100259291270526No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTA70.12100259291270526No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGTCGCC70.12100259291270526Illumina Single End PCR Primer 1 (95% over 22bp)
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.12100259291270526No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT70.12100259291270526No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGG70.12100259291270526No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT70.12100259291270526No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA60.10371650821089023No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA60.10371650821089023No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGGC60.10371650821089023No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT60.10371650821089023No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA60.10371650821089023No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTC60.10371650821089023No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA60.10371650821089023No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA60.10371650821089023No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG60.10371650821089023No Hit
GTGTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCT60.10371650821089023No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGGGCC60.10371650821089023No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAA60.10371650821089023No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA60.10371650821089023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)