FastQCFastQC Report
Tue 7 May 2024
EGAF00007822320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13643
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT7525.51198416770505No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTA5934.346551345012093No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4683.430330572454739No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGA1381.0115077329033204No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTT1340.982188668181485No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.608370592978084No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGG800.5863812944367075No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC700.513083632632119No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTG590.43245620464707174No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGT550.40313713992523637No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAG540.3958073737447775No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.3371692443011068No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA380.2785311148574361No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC360.2638715824965184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG350.2565418163160595No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG300.2198929854137653No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT290.21256321923330646No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT280.2052334530528476No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG270.19790368687238874No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC270.19790368687238874No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.18324415451147108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC240.17591438833101225No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC210.1539250897896357No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGG210.1539250897896357No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.1539250897896357No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTC200.14659532360917688No Hit
CTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTCGG200.14659532360917688No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.13926555742871805No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.13926555742871805No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAG190.13926555742871805No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13926555742871805No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA170.12460602506780033No Hit
AGCTTGTATAGTACCAAAGCCGGCAAAATTCGCCGGACAATACCTCCACT170.12460602506780033No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC170.12460602506780033No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1172762588873415No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT160.1172762588873415No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG160.1172762588873415No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA150.10994649270688266No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA140.1026167265264238No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA140.1026167265264238No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAG140.1026167265264238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)