FastQCFastQC Report
Tue 7 May 2024
EGAF00007822321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5278
Total Bases796.9 kbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1873.5430087154225083No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1112.103069344448655No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA711.3452065176203107No Hit
ATTGCGCAATGGGGGCACAGGGGTGAAAAATTGGAAAGGAGTAGAAGAGA350.6631299734748011No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG300.5683971201212581No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.378931413414172No Hit
ATTGCGCAATGAATCCACGGGGGTAGCGATAAAGGGAGTGAAAAGCTCCC180.34103827207275483No Hit
ATTGCGCAATGAATAAAGGGGGGTACGTGAGCTGGGTTTAGAACGTCGTG170.32209170140204624No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG160.30314513073133764No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG150.28419856006062905No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTC140.2652519893899204No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.2652519893899204No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA110.2084122773777946No Hit
ATTGCGCAATGGTGGGGCTGGGGATTTCCTTGCCCCTTTGAGGGTGTGAG100.189465706707086No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.17051913603637742No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG90.17051913603637742No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT80.15157256536566882No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC70.1326259946949602RNA PCR Primer, Index 40 (96% over 29bp)
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT70.1326259946949602No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATGG70.1326259946949602No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC60.11367942402425162No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA60.11367942402425162No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT60.11367942402425162No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAT60.11367942402425162No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA60.11367942402425162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)