FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5278
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2574.869268662372111No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1542.9177718832891246No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGT1162.197802197802198No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT771.4588859416445623No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGG561.0610079575596816No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.7768093974990526No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTT270.5115574081091322No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC260.49261083743842365No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG220.4168245547555892No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.4168245547555892No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGA200.378931413414172No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTA200.378931413414172No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATC200.378931413414172No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA180.34103827207275483No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.34103827207275483No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTG160.30314513073133764No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG150.28419856006062905No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC150.28419856006062905No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.28419856006062905No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC150.28419856006062905No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTG140.2652519893899204No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG130.24630541871921183No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG120.22735884804850323No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.22735884804850323No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA110.2084122773777946No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC110.2084122773777946No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA100.189465706707086No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC100.189465706707086No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT90.17051913603637742No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGG90.17051913603637742No Hit
GTATAGAGGTACAGGAATATTAACCTGTTTCCCATCAGCTACGCATCTCT90.17051913603637742No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC90.17051913603637742No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC90.17051913603637742No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC80.15157256536566882No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.15157256536566882No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGG70.1326259946949602No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA70.1326259946949602No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGT70.1326259946949602No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA70.1326259946949602No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAA70.1326259946949602No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG70.1326259946949602No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA70.1326259946949602No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.11367942402425162No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATGT60.11367942402425162No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGGGGGGG60.11367942402425162No Hit
CCTTGTTTTGAGGGGGGCTTCACGCTTAGATGCCTTCAGCGTTTATCCTG60.11367942402425162No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAA60.11367942402425162No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGG60.11367942402425162No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTG60.11367942402425162No Hit
GCATACGCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG60.11367942402425162No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGGTGGTCGCC60.11367942402425162Illumina RNA PCR Primer (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)