FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4391
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2866.513322705534047No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1473.347756775222045No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT551.2525620587565476No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC320.7287633796401731No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.5465725347301298No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATC220.501024823502619No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGT200.4554771122751082No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.36438168982008656No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG150.3416078342063311No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGGTGGTCGCC140.31883397859257573Illumina RNA PCR Primer (95% over 24bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG100.2277385561375541No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC100.2277385561375541No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGG100.2277385561375541No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG100.2277385561375541No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGG90.20496470052379867No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG90.20496470052379867No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC90.20496470052379867No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG90.20496470052379867No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.18219084491004328No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGG70.15941698929628786No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGGGGG70.15941698929628786No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA70.15941698929628786No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15941698929628786No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTG70.15941698929628786No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC60.13664313368253245No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGGGGGGG60.13664313368253245No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.13664313368253245No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTA60.13664313368253245No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTG60.13664313368253245No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.13664313368253245No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA60.13664313368253245No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTCGGGGGGGGGG60.13664313368253245No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGGGGGGGGGG60.13664313368253245No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC60.13664313368253245No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA50.11386927806877704No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT50.11386927806877704No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGGGGGGGGGG50.11386927806877704No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGG50.11386927806877704No Hit
TCCTTAGAGTGCCCAACTGAATGATGGCAACTAGGGATGAGGGTTGCGCT50.11386927806877704No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTT50.11386927806877704No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATG50.11386927806877704No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGGGTGGG50.11386927806877704No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC50.11386927806877704No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG50.11386927806877704No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC50.11386927806877704No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGG50.11386927806877704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)