FastQCFastQC Report
Tue 7 May 2024
EGAF00007822334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412
Total Bases213.2 kbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT18813.314447592067987No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC775.453257790368272No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC181.2747875354107647No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171.2039660056657222No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGGTGGTCGCC90.6373937677053824Illumina RNA PCR Primer (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGG80.56657223796034No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGGGGGGGGGG80.56657223796034No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.49575070821529743No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTGGG60.424929178470255No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.424929178470255No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGGGGGGG50.3541076487252125No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTG50.3541076487252125No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTGGGGGGGGGGG50.3541076487252125No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGG50.3541076487252125No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA40.28328611898017No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGT40.28328611898017No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGGGGGGGGGG40.28328611898017No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG40.28328611898017No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGG40.28328611898017No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTGGTGGG40.28328611898017No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGGGGGGGG30.2124645892351275No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTCGG30.2124645892351275No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGG30.2124645892351275No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCGTGCTGATCCACGAT30.2124645892351275No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTCGGGGGGGGGG30.2124645892351275No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGGGGG30.2124645892351275No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGG30.2124645892351275No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGGGGGGGGGG30.2124645892351275No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2124645892351275No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA30.2124645892351275No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGCGGGGGGGGGG30.2124645892351275No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTCGG30.2124645892351275No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTGGGGGG30.2124645892351275No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTATGGG20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGGGGGGC20.141643059490085No Hit
TCACAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGGGTGGGGGGGGGGG20.141643059490085No Hit
ATATTATACATATGTAGTAATACTTAGGGAGTTATTATTAATGTATCATA20.141643059490085No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTGGGTGG20.141643059490085No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTC20.141643059490085No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTG20.141643059490085No Hit
GTCCCCTCCTCATGGCTATGTGTTCACCATGGGATGCTTAAGTATTACCT20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTGGGTGTGGGGGGGGGGG20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGGGGGGGGGGGGG20.141643059490085No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTC20.141643059490085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.141643059490085No Hit
GTATAAGAGACAGATTGAGCAATCTGTCTCTTATACACATCTGACGCTGC20.141643059490085No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAAAT20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTGGGGGGGGGGGGGG20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTCGGGGGGGGGG20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTATATATCGGTGGTCGCC20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGGGGGGGGGC20.141643059490085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA20.141643059490085No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTGGGTGTGGG20.141643059490085No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGG20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGGGGGGGGGGGGGGG20.141643059490085No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGGTGG20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTCGCGGGGGGGGGG20.141643059490085No Hit
AGATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGA20.141643059490085No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGAGGTGTGGGGGGGGGGG20.141643059490085No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCAAAAATTAA20.141643059490085No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGG20.141643059490085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC20.141643059490085No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCC20.141643059490085No Hit
GTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATC20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTCGGTGGGGGGG20.141643059490085No Hit
GTATCATATTTTATTACTATTACATTATATGAAATTATATACTATATAAA20.141643059490085No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATTTAT20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGTGGGGGGG20.141643059490085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTAGGGGGGGGGG20.141643059490085No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGGGGG20.141643059490085No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTCGG20.141643059490085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)