FastQCFastQC Report
Tue 7 May 2024
EGAF00007822338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5571
Total Bases841.2 kbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3957.090288996589482No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2254.038772213247173No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGT761.3642075031412673No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC390.7000538502961766No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.5923532579429186No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.5385029617662896No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGG290.5205528630407468No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTT240.4308023694130318No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG240.4308023694130318No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATC210.3769520732364028No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTA190.3410518757853168No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC190.3410518757853168No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTG180.32310177705977383No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC160.28720157960868786No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.2513013821576019No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGA130.23335128343205885No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG130.23335128343205885No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG120.2154011847065159No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA120.2154011847065159No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGG100.1795009872554299No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTG100.1795009872554299No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG100.1795009872554299No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC100.1795009872554299No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG100.1795009872554299No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGG100.1795009872554299No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGGGGG100.1795009872554299No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA100.1795009872554299No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA100.1795009872554299No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC100.1795009872554299No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC90.16155088852988692No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGG90.16155088852988692No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.16155088852988692No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.16155088852988692No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC90.16155088852988692No Hit
GTACAGGATCACTCACTCACCCACAACCATGGGCTCCCCATCATGCCTCA80.14360078980434393No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA80.14360078980434393No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGGGGG80.14360078980434393No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTG80.14360078980434393No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTG80.14360078980434393No Hit
CCCCAGAAGCATCAAAAACACTCCCAAGACAAGCTCACATGCTTAGCATC80.14360078980434393No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGGTGGTCGCC80.14360078980434393Illumina RNA PCR Primer (95% over 24bp)
GTACAGGATCACTCACTCACCCACAACCATGGGCTCCCCAACATGCCTCA70.12565069107880095No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTC70.12565069107880095No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.12565069107880095No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAGTGT60.10770059235325795No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGGGGGGG60.10770059235325795No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGG60.10770059235325795No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.10770059235325795No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTG60.10770059235325795No Hit
CAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG60.10770059235325795No Hit
CTGTAGCTGTCTTCAGACACCCCAGAAGAGGGCATCAGATCTCATTATGG60.10770059235325795No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTGGG60.10770059235325795No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT60.10770059235325795No Hit
TCTTCATACTATACAATTACATTGGTTATTCTGTTTTAAATTTAATTCAC60.10770059235325795No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA60.10770059235325795No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGG60.10770059235325795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)