FastQCFastQC Report
Tue 7 May 2024
EGAF00007822343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8192
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCG3314.04052734375No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCT2432.96630859375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1401.708984375No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1061.2939453125No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1051.28173828125No Hit
ATTGCGCAATGCGGGGCATGGGGGGGAAAACCTCTCTATTCAGCACTTCC871.06201171875No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTAC450.54931640625No Hit
ATTGCGCAATGGTCCACGAGGGGCGCCTGAATGTATAGGGCGTGTGGAGG430.52490234375No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAT380.4638671875No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC360.439453125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT330.40283203125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG330.40283203125No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAA320.390625No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240.29296875No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.28076171875No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCG210.25634765625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT200.244140625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT190.23193359375No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAGCG150.18310546875No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAA150.18310546875No Hit
ATTGCGCAATGTACCAGTTGGGGGCTAACTATCCAAAGAGAGAATAAACC140.1708984375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT140.1708984375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC130.15869140625No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCT130.15869140625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.15869140625No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA130.15869140625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC120.146484375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG120.146484375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC120.146484375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.13427734375No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCGCG110.13427734375No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG100.1220703125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG100.1220703125No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATGG100.1220703125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT100.1220703125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC90.10986328125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG90.10986328125No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTCCGATCG90.10986328125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT90.10986328125No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA90.10986328125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)