FastQCFastQC Report
Tue 7 May 2024
EGAF00007822345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7218
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG3154.364089775561098No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCT2403.3250207813798838No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1472.036575228595179No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1401.9395954558049322No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC731.0113604876697146No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC360.4987531172069825No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAT360.4987531172069825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG350.48489886395123305No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTG330.457190357439734No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC330.457190357439734No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT230.31864782488223886No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.2770850651149903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT200.2770850651149903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA180.24937655860349126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT170.23552230534774174No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG170.23552230534774174No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG160.22166805209199225No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAGCG150.20781379883624274No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA150.20781379883624274No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.20781379883624274No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG140.19395954558049322No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC130.18010529232474368No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA120.1662510390689942No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATGG120.1662510390689942No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTG120.1662510390689942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.15239678581324467No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG110.15239678581324467No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCA110.15239678581324467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG100.13854253255749516No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT100.13854253255749516No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG100.13854253255749516No Hit
ATTGCGCAATGCGAAACGGGGGAGAACAGACAAAAATAAAAAAACAAACA100.13854253255749516No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC90.12468827930174563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC90.12468827930174563No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCCTCG90.12468827930174563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC80.11083402604599613No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCT80.11083402604599613No Hit
ATTGCGCAAAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAG80.11083402604599613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGTGCCTG80.11083402604599613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT80.11083402604599613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT80.11083402604599613No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC80.11083402604599613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGGTG80.11083402604599613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT80.11083402604599613No Hit
ATTGCGCAATGTATGCTGCTGCTGTCTCTTATACACATCTCCGAGCCCAC80.11083402604599613No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCGCG80.11083402604599613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)