FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7218
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2173.006372956497645No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGT1802.493765586034913No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGG1472.036575228595179No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1191.6486561374341921No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.8589637018564699No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.8174009420892214No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTA410.5680243834857301No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT380.5264616237184816No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTT340.4710446106954835No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG340.4710446106954835No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTG330.457190357439734No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTG310.42948185092823493No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGA290.4017733444167359No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC270.3740648379052369No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG260.36021058464948735No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.3463563313937379No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTG240.3325020781379884No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.29093931837073983No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG180.24937655860349126No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC180.24937655860349126No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC180.24937655860349126No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG170.23552230534774174No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC160.22166805209199225No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGT160.22166805209199225No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.22166805209199225No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC150.20781379883624274No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.19395954558049322No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA130.18010529232474368No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG130.18010529232474368No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA130.18010529232474368No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA120.1662510390689942No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT110.15239678581324467No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT100.13854253255749516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA100.13854253255749516No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCT100.13854253255749516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC100.13854253255749516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.13854253255749516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA100.13854253255749516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA90.12468827930174563No Hit
CTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGGGGTGTGGG90.12468827930174563No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCGGGTGGG90.12468827930174563No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.12468827930174563No Hit
CATCTGACGCTGCCGACGACGCTTATCTGGTGGGGGTGTGGGGGGGGGGG90.12468827930174563No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC90.12468827930174563No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA80.11083402604599613No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGGGGT80.11083402604599613No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGGGGG80.11083402604599613No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT80.11083402604599613No Hit
TTTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG80.11083402604599613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)