FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9688
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG2542.6218001651527665No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCT2292.3637489677952104No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2132.198596201486375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1911.9715111478117258No Hit
ATTGCGCAATGCGTGCTACGGGGCGCAACGTCCGAAAGCGACGGCTGAAA1751.8063583815028903No Hit
ATTGCGCAATGAGGCAGGGGGGAAGTTTCCAAGGGCGCATGGTGGATGCC1431.4760528488852187No Hit
ATTGCGCAATGCTCTCAAAGGGGCCTTTTGTGACTGGCGGTGAACGAGTG1391.43476465730801No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1321.3625103220478945No Hit
ATTGCGCAATGAAGCCTGTGGGATCGCCCGCGGCGCCAGCAACTTTGTCA1091.1251032204789428No Hit
ATTGCGCAATGCTTTACAAGGGGCCTTTTGTGACTGGCGGTGAACGAGTG1021.0528488852188274No Hit
ATTGCGCAATGTTTGGACGGGGGGGAATTGATGCAGGTGGTGTTACTCGA950.9805945499587119No Hit
ATTGCGCAATGGTGGTTTCGGGGGCGTAAATCTAACTTTCTTCCCACAAC920.9496284062758051No Hit
ATTGCGCAATGCCAATTGGGGGGTCACTCCTTCGCCGTTGTGCCGAGCCC920.9496284062758051No Hit
ATTGCGCAATGAGGAGGCTGGGGTCGCGCGGGCCCGTGGTGTGGACCCGC670.6915772089182494No Hit
ATTGCGCAATGCAGGCGACGGGAACGTGGCGCCTGATGCGCGCGCCCTGC660.6812551610239472No Hit
ATTGCGCAATGTATTGGCTGGGCAGGTGGGATGGTAGAGTATACCAAGGC590.6090008257638315No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG480.495458298926507No Hit
ATTGCGCACTGAGGGTCGCGGGTCCATCCAAAACCGATAAATCTTTCCAA420.4335260115606936No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAAT340.3509496284062758No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTG340.3509496284062758No Hit
ATTGCGCAATGAAGGCCAAGAAGGAGGCGCCGCCCATGGAGAAGCCGGAA320.3303055326176713No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC320.3303055326176713No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTA320.3303055326176713No Hit
ATTGCGCAATGGATTAACCGGGGGGGCCGATCGGTTTCGACGGTGTGTGT310.3199834847233691No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT270.2786952931461602No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC260.26837324525185796No Hit
ATTGCGCAATGTATAAGGGGGGGCTTTCTATCAAAAGCAGCCTTATAAAA260.26837324525185796No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT230.23740710156895128No Hit
ATTGCGCAATGGGGGGGTTGGGGAGATGGCTCAGTGGTTAAGAGCACCGA220.22708505367464907No Hit
ATTGCGCAATGTCTGATCCGGGGGGGTTGGCTCTTGTCTATGTTTCTTGG180.18579686209744012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT170.1754748142031379No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT160.16515276630883566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.15483071841453344No Hit
ATTGCGCAATGCGTCGAGGGGGGATACCCAAACCAAAAACAAACAAAAAA150.15483071841453344No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA140.1445086705202312No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG140.1445086705202312No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTGCGTTAAATCG140.1445086705202312No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC130.13418662262592898No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG120.12386457473162675No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA120.12386457473162675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC110.11354252683732453No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC110.11354252683732453No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTGCGGTAAATCG110.11354252683732453No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT110.11354252683732453No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA110.11354252683732453No Hit
ATTTTAGTCTTTGCTACAATAAATGCTATATTTCTTTTAAACAAGCCTAA110.11354252683732453No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC110.11354252683732453No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT100.10322047894302229No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG100.10322047894302229No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTGT100.10322047894302229No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT100.10322047894302229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)