FastQCFastQC Report
Tue 7 May 2024
EGAF00007822357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366
Total Bases357.2 kbp
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1737.311918850380389No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC943.9729501267962806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.84530853761623No Hit
ATTGCGCAATGTCAGCCTAGGGGGGCCCATAGCTCAGTTGGTAGAGCAAC190.8030431107354183No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG170.7185122569737954No Hit
ATTGCGCAATGGTTGGGAGGGGGGGCGAAAGCCTGATCCAGCAATGCCGC110.46491969568892644No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.422654268808115No Hit
ATTGCGCAATGCGGCACCTGGGCGAAAGACTAAGGTTTCCTCAGCCATGC70.2958579881656805No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.2113271344040575No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.16906170752324598No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC40.16906170752324598No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC30.12679628064243448RNA PCR Primer, Index 40 (96% over 29bp)
ATTGCGCAATCTGTCTCTTATACACCGGTCTCTTATACACATCTCCGAGC30.12679628064243448No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG30.12679628064243448No Hit
ATTGCGCAAACTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC30.12679628064243448No Hit
ATTGCGCAATGGTCCTGGGGGGTCGGTTGTGTATTTATCCTCGCCCACAA30.12679628064243448No Hit
GGGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.12679628064243448No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCACCGAGCCC30.12679628064243448No Hit
GCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.12679628064243448No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCACCGAGC30.12679628064243448No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)