FastQCFastQC Report
Tue 7 May 2024
EGAF00007822358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366
Total Bases357.2 kbp
Sequences flagged as poor quality0
Sequence length151
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2369.974640743871513No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1395.874894336432798No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC230.9721048182586645No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.84530853761623No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG190.8030431107354183No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT180.760777683854607No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG160.6762468300929839No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG160.6762468300929839No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG140.591715976331361No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.5494505494505495No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGG110.46491969568892644No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGG110.46491969568892644No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.46491969568892644No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG110.46491969568892644No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG110.46491969568892644No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGG100.422654268808115No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAAC90.3803888419273035No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG80.33812341504649196No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTG80.33812341504649196No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGG70.2958579881656805No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.2958579881656805No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG70.2958579881656805No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTT70.2958579881656805No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGG60.25359256128486896No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC60.25359256128486896No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGG50.2113271344040575No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.2113271344040575No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG50.2113271344040575No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGGGGGGG50.2113271344040575No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTG50.2113271344040575No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGG50.2113271344040575No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG50.2113271344040575No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGGGGGGG50.2113271344040575No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG50.2113271344040575No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGG50.2113271344040575No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC40.16906170752324598No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGG40.16906170752324598No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGG40.16906170752324598No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCT40.16906170752324598No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGGGGGGGGG40.16906170752324598No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTG40.16906170752324598No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA40.16906170752324598No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.16906170752324598No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGG40.16906170752324598No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGGGGGGGGGG40.16906170752324598No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTG40.16906170752324598No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTG40.16906170752324598No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC40.16906170752324598No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC40.16906170752324598No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGG30.12679628064243448No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA30.12679628064243448No Hit
CAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG30.12679628064243448No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAATATG30.12679628064243448No Hit
GCATACGACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC30.12679628064243448No Hit
TCCTTAGGATTATCAGCGCATCCGAAGACTTGCTGTACTATCGTAACCTC30.12679628064243448No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC30.12679628064243448No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA30.12679628064243448No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT30.12679628064243448No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGG30.12679628064243448No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTG30.12679628064243448No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTC30.12679628064243448No Hit
GTATAAATCGCTTTTCTTGGAAGCGGGTCCTTAGGATTATCAGCGCATCC30.12679628064243448No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGGGGGGG30.12679628064243448No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTG30.12679628064243448No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGGGGGGG30.12679628064243448No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGA30.12679628064243448No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGG30.12679628064243448Illumina RNA PCR Primer (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTA30.12679628064243448No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTG30.12679628064243448No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGG30.12679628064243448No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATATCGGTGG30.12679628064243448No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGG30.12679628064243448No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGG30.12679628064243448No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA30.12679628064243448No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCC30.12679628064243448Illumina RNA PCR Primer (96% over 29bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA30.12679628064243448No Hit
ATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA30.12679628064243448No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA30.12679628064243448No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTG30.12679628064243448No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)