FastQCFastQC Report
Tue 7 May 2024
EGAF00007822364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723
Total Bases260.1 kbp
Sequences flagged as poor quality0
Sequence length151
%GC63

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT21812.6523505513639No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC804.643064422518862No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG211.2188044109112013No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG181.044689495066744No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.8705745792222866No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC140.812536273940801No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG140.812536273940801No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG120.6964596633778294No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT90.522344747533372No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGG70.4062681369704005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.4062681369704005No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGG70.4062681369704005No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTG70.4062681369704005No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGG70.4062681369704005No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGG60.3482298316889147No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG60.3482298316889147No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGGGGGGG60.3482298316889147No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGGGGGGG60.3482298316889147No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCC60.3482298316889147Illumina RNA PCR Primer (96% over 29bp)
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG50.2901915264074289No Hit
CTATAAAGGTATCCACAGCTTCGGTAAATTGTTTTAGCCCCGGTACATTT50.2901915264074289No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTG50.2901915264074289No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG50.2901915264074289No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG50.2901915264074289No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGG50.2901915264074289No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC50.2901915264074289No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGG40.2321532211259431No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGG40.2321532211259431No Hit
GGATTCTCACCTGTCTTTCGCTACTCATACCGGCATTCTCACTTCTATGC40.2321532211259431No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTC40.2321532211259431No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGG40.2321532211259431No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA40.2321532211259431No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTG40.2321532211259431No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAAC40.2321532211259431No Hit
GTCATATACGAGTATTTAGGCTTAGAGGGTGGTCCCCCTATGTTCAGACA40.2321532211259431No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGG40.2321532211259431Illumina RNA PCR Primer (95% over 21bp)
GTACTGGTTCACTATCGGTCGCTGAGGAGTACTTAGGCTTGGAGGGTGGT40.2321532211259431No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGGGGGGGGGG30.17411491584445735No Hit
GTGTATAAGAGACAGATTGCGCACTCTGTCTCTTATACACATCTGACGCT30.17411491584445735No Hit
TGTGTATACAGTTACATGGAACCTTCAGGTCAGTGGGGCACAAATGTGGT30.17411491584445735No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA30.17411491584445735No Hit
GTACTAGTTCGCTATCGGTCATATACGAGTATTTAGGCTTAGAGGGTGGT30.17411491584445735No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCT30.17411491584445735No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGTGGGGGGG30.17411491584445735No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTCGGGGGGGGGG30.17411491584445735No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGGGGGGGGGGGGG30.17411491584445735No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTG30.17411491584445735No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGG30.17411491584445735No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTG30.17411491584445735No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGTGGGG30.17411491584445735No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGGGGGGG30.17411491584445735No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGGGGGGG30.17411491584445735No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTG30.17411491584445735No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGG30.17411491584445735No Hit
ATTTTGGAGTCTGGATTCTTCACTTCCAGCTGGTTGAATATAAACTCAAT30.17411491584445735No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGGGGGGG30.17411491584445735No Hit
CTCTCCTACCATACCTATAAAGGTATCCACAGCTTCGGTAAATTGTTTTA20.11607661056297155No Hit
GGTATACACACGATGAGCCTGGCAGGGAAGCAAATGAGACCAGGCTTGGA20.11607661056297155No Hit
CACGATGAGCCTGGCAGGGAAGCAAATGAGACCAGGCTTGGAGCTACAGG20.11607661056297155No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGTGGGGGGG20.11607661056297155No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGTGGTGGGG20.11607661056297155No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11607661056297155No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.11607661056297155No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG20.11607661056297155No Hit
TATCACACCTCACTGCTTAGACAGACACTTCCAATCGTCTGCTTTAGTTA20.11607661056297155No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGTGG20.11607661056297155No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA20.11607661056297155No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGGGGG20.11607661056297155No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGC20.11607661056297155No Hit
ATTCATGTGTATACAGTTACATGGAACCTTCAGGTCAGTGGGGCACAAAT20.11607661056297155No Hit
GTGGTCCCCCTATGTTCAGACAGAATTACACGTGTTCCGCCCTACTCGAG20.11607661056297155No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGG20.11607661056297155No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTGGGTCTCGGGGGGGGGG20.11607661056297155No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAATATT20.11607661056297155No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGCTCGGGGG20.11607661056297155No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.11607661056297155No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGGGGGGG20.11607661056297155No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGG20.11607661056297155No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11607661056297155No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGG20.11607661056297155No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGGGGG20.11607661056297155No Hit
GATGTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAA20.11607661056297155No Hit
GTGTAAAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT20.11607661056297155No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGTTCTCGG20.11607661056297155No Hit
TTCCAGCACTCCTCACGGTACACCTTCTTCACACATAGAACGCTCTCCTA20.11607661056297155No Hit
CTCTTTGAATGAATAGCTGCTTCTAAGCTAACATCCTAGTTGTCTGTGCA20.11607661056297155No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTCGG20.11607661056297155No Hit
TCTCACTTCTATGCGTTCCAGCACTCCTCACGGTACACCTTCTTCACACA20.11607661056297155No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11607661056297155No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTA20.11607661056297155No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT20.11607661056297155No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGTGGGGGGG20.11607661056297155No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTGGGGGTG20.11607661056297155No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGG20.11607661056297155No Hit
ATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACG20.11607661056297155No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA20.11607661056297155No Hit
CCTCACGGTACACCTTCTTCACACATAGAACGCTCTCCTACCATACCTAT20.11607661056297155No Hit
ACCTTCAGGTCAGTGGGGCACAAATGTGGTATACACACGATGAGCCTGGC20.11607661056297155No Hit
GCATTTAAGTATTCATGTGTATACAGTTACATGGAACCTTCAGGTCAGTG20.11607661056297155No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGG20.11607661056297155No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTTGGTGTC20.11607661056297155No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG20.11607661056297155No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGGGGTGGGGGGG20.11607661056297155No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGG20.11607661056297155No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGTGTGTGGG20.11607661056297155No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAT20.11607661056297155No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGGGGGGGGGG20.11607661056297155No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.11607661056297155No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGTGGG20.11607661056297155No Hit
GGCTTGGAGGGTGGTCCCCCCATGTTCAGACAGGATTTCACGTGTCCCGC20.11607661056297155No Hit
CTTTCTGAAGAAAAGAAGACTCAATGGAAACAGCCTTTCTTGCTTTGTAG20.11607661056297155No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAATAT20.11607661056297155No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTG20.11607661056297155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)