FastQCFastQC Report
Tue 7 May 2024
EGAF00007822373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11427
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG5004.375601645226219No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT3242.8353898661065897No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2191.916513520609084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1881.6452262186050584No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1551.3564365100201279No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAT770.6738426533648377No Hit
ATTGCGCAATGTTTCGGGGAGGCGGCAATATCCTGTGATCCGGAGATTTC750.6563402467839329No Hit
ATTGCGCAATGAGCCCAGTGGGGAGTGTTCAAGAGCGCATGGTGGATGCC620.5425746040080511No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTC620.5425746040080511No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC620.5425746040080511No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA530.46381377439397914No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT510.4463113678130743No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG490.42880896123216944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT460.40255535136081205No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC320.280038505294478No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC310.27128730200402557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT270.2362824888422158No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.22753128555176336No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA240.2100288789708585No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG230.20127767568040603No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.20127767568040603No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG220.1925264723899536No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.1925264723899536No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAG210.18377526909950118No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCG200.17502406580904875No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT190.16627286251859633No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA190.16627286251859633No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.15752165922814387No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.14877045593769145No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGT170.14877045593769145No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC150.13126804935678654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.13126804935678654No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA150.13126804935678654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG150.13126804935678654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.12251684606633412No Hit
ATTGCGCAATGTGTAACGAGGGAGCACACCGAAAAGAAGAAAATTATAAA130.11376564277588168No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG130.11376564277588168No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT120.10501443948542925No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAAGCG120.10501443948542925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGTC120.10501443948542925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT120.10501443948542925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGCTGTCTCTTA120.10501443948542925No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGAGAGTCAATCT120.10501443948542925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)