FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11427
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTA8537.464776406755928No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2892.529097750940754No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1871.636475015314606No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.7963594994311717No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.761354686269362No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGG800.700096263236195No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTG770.6738426533648377No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACA580.5075697908462413No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA520.4550625711035267No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.3412969283276451No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG380.33254572503719265No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTC370.32379452174674017No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC350.3062921151658353No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA340.2975409118753829No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG340.2975409118753829No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC320.280038505294478No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC320.280038505294478No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.280038505294478No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC270.2362824888422158No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG260.22753128555176336No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTCGG260.22753128555176336No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA240.2100288789708585No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA230.20127767568040603No Hit
CATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGGTCGCC230.20127767568040603Illumina Single End PCR Primer 1 (100% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC220.1925264723899536No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC220.1925264723899536No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGA210.18377526909950118No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.17502406580904875No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACTGTGTA190.16627286251859633No Hit
ATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGG180.15752165922814387No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.15752165922814387No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCAC170.14877045593769145No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAG170.14877045593769145No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.14877045593769145No Hit
CATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGGGCGCC160.140019252647239Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.140019252647239No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA160.140019252647239No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC150.13126804935678654No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC150.13126804935678654No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGGGTA140.12251684606633412No Hit
CCCTATATATTCAGATGCGGGTCACGACACACAACATGCCGCGGGGTTTC140.12251684606633412No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA140.12251684606633412No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG140.12251684606633412No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA130.11376564277588168No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA130.11376564277588168No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC130.11376564277588168No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGA130.11376564277588168No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT130.11376564277588168No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA130.11376564277588168No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA130.11376564277588168No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC130.11376564277588168No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG120.10501443948542925No Hit
GTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATC120.10501443948542925No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTT120.10501443948542925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)