FastQCFastQC Report
Tue 7 May 2024
EGAF00007822375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9637
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCG4024.171422641901006No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCT2722.822455120888243No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2102.1791013800975407No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1601.6602677181695547No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1181.2244474421500466No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTAC670.695237106983501No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC660.6848604337449413No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG580.6018470478364637No Hit
ATTGCGCAATGCTCCGGGGGGGGTAGACGCGAAACTTTGTGAGCTACCCT570.591470374597904No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAT500.518833661927986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT450.4669502957351873No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC430.4461969492580679No Hit
ATTGCGCAATGGGGGACGAGGGGGGTGAGAGTCCTGTAACTGTAAGCGTG400.41506692954238866No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAA350.3631835633495901No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT330.34243021687247066No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA310.32167687039535126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT310.32167687039535126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA280.2905468506796721No Hit
ATTGCGCAATGGAAGACGAGGGGGGTGAGAGTCCTGTAACTGTAAGCGTG270.2801701774411124No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCG250.259416830963993No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.259416830963993No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT220.2282868112483138No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT190.19715679153263466No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG190.19715679153263466No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC180.18678011829407493No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAA170.1764034450555152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG170.1764034450555152No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCT160.1660267718169555No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.1660267718169555No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.15565009857839576No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG150.15565009857839576No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAG140.14527342533983606No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.14527342533983606No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC140.14527342533983606No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAGCT140.14527342533983606No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT140.14527342533983606No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC130.13489675210127633No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT130.13489675210127633No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG130.13489675210127633No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGATCGCG130.13489675210127633No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAAGGTACGAGCG130.13489675210127633No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC130.13489675210127633No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG130.13489675210127633No Hit
ATTGCGCAATGATAGGGGGGGGGTAGACGCGAAACTTTGTGAGCTACCCT120.12452007886271661No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT120.12452007886271661No Hit
ATTGCGCAATGCGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAG120.12452007886271661No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT110.1141434056241569No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT110.1141434056241569No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA100.10376673238559717No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT100.10376673238559717No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT100.10376673238559717No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT100.10376673238559717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)