FastQCFastQC Report
Tue 7 May 2024
EGAF00007822376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9637
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTA5025.209089965756979No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2652.7498184082183252No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1591.6498910449309951No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.7471204731762997No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACA590.6122237210750233No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.5603403548822248No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGG490.5084569886894261No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTG390.40469025630382904No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC380.3943135830652693No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACTGTGTA310.32167687039535126No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTC290.30092352391823185No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA270.2801701774411124No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG270.2801701774411124No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC260.26979350420255266No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC250.259416830963993No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA240.24904015772543323No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.2282868112483138No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.2282868112483138No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA210.2179101380097541No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTT200.20753346477119433No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.20753346477119433No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.19715679153263466No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC160.1660267718169555No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG150.15565009857839576No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.15565009857839576No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC140.14527342533983606No Hit
CATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGGTCGCC140.14527342533983606Illumina Single End PCR Primer 1 (100% over 22bp)
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA130.13489675210127633No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGGA130.13489675210127633No Hit
CATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGGGCGCC120.12452007886271661Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.12452007886271661No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTCGG120.12452007886271661No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA120.12452007886271661No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG120.12452007886271661No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACGGTGTA110.1141434056241569No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG110.1141434056241569No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA110.1141434056241569No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGGGTA100.10376673238559717No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC100.10376673238559717No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC100.10376673238559717No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA100.10376673238559717No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10376673238559717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)