FastQCFastQC Report
Tue 7 May 2024
EGAF00007822377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9685
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG4554.697986577181208No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCT3523.6344863190500774No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1791.848218895198761No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1761.8172431595250387No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1081.1151264842540012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG620.6401652039235932No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAT530.5472379969024264No Hit
ATTGCGCAATGCGCGGACAGGGGGGGAAGAGGTAGAAAAAGGGAGGGTGA510.5265875064532782No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTG510.5265875064532782No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC490.5059370160041301No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC430.4439855446566856No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT380.39235931853381517No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT330.34073309241094474No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCG260.2684563758389262No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT250.2581311306143521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA240.24780588538977802No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC240.24780588538977802No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240.24780588538977802No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG210.21683014971605577No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAGCG200.20650490449148168No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA180.1858544140423335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.1858544140423335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG180.1858544140423335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC160.16520392359318534No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTG150.15487867836861124No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.15487867836861124No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG150.15487867836861124No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCAGCT140.14455343314403718No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATGG140.14455343314403718No Hit
ATTGCGCAATGAAGAAAGGGGGGGAAAAATGAAATGTGAATAAAAAAGGA130.1342281879194631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT130.1342281879194631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT130.1342281879194631No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGT130.1342281879194631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGTC120.12390294269488901No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT120.12390294269488901No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC110.11357769747031492No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT110.11357769747031492No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG110.11357769747031492No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT110.11357769747031492No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA100.10325245224574084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT100.10325245224574084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG100.10325245224574084No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGC100.10325245224574084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG100.10325245224574084No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTGCAAGTGCATCGCG100.10325245224574084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT100.10325245224574084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)