FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5718
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1843.217908359566282No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1071.8712836656173488No Hit
ATTGCGCAATGACTCGACCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTG951.6614200769499825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA891.5564882826162993No Hit
ATTGCGCAATGAACAGAACGGGGGTGGTTGGACTGATATGGTTACGGTGG681.1892270024484084No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG540.944386149003148No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCG500.8744316194473591No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT280.48968170689052115No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGC270.472193074501574No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.3497726477789437No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG180.3147953830010493No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG170.2973067506121021No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA170.2973067506121021No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG170.2973067506121021No Hit
ATTGCGCAATGGGCGAAACGGGAAGGTGGACAAGAAGAAGAAAAAGAAGG160.27981811822315494No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATCT150.26232948583420773No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAT140.24484085344526058No Hit
ATTGCGCAATGCTGAGGTAGGGACAAAAAAAAGGCATATAAAAAAAAGTC120.2098635886673662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC110.19237495627841902No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT100.17488632388947184No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG90.15739769150052466No Hit
ATTGCGCAATGATGAGGCAGGGACAAAAAAAAGGCATATAAAAAAAAGTC90.15739769150052466No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.13990905911157747No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.13990905911157747No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT70.12242042672263029No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA70.12242042672263029No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT60.1049317943336831No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC60.1049317943336831No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCACTCG60.1049317943336831No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC60.1049317943336831No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAATGG60.1049317943336831No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC60.1049317943336831No Hit
ATTGCGCAATGTACAATCCGGGGTAATGAAACTGGATCCAAGAAAAGCCG60.1049317943336831No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCACGCAAGCG60.1049317943336831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)