FastQCFastQC Report
Tue 7 May 2024
EGAF00007822387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9797
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATGAATACCACGGGCCTTTTGTGACTGGCGGTGAACGAGTGC3543.613351025824232No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG3263.3275492497703376No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCT2862.919260998264775No Hit
ATTGCGCAATGTCAGATCGGGGGGGAAGATCAGCCAAAGAGAGTAATAGC2182.2251709707053178No Hit
ATTGCGCAATGTTCGCGTGGGTCAAACTTGAGAGTTTGATCCTGGCTCAG2022.0618556701030926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1911.949576400939063No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1571.6025313871593345No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1391.4188016739818312No Hit
ATTGCGCAATGGCTATAGGGGGGGGCCCCTCCTTGTCATGTCTGCCGGCA1111.1329998979279372No Hit
ATTGCGCAATGGTAGCATGGGGAACGATGAGGTACTGATCGAAGGTTTCG960.9798918036133509No Hit
ATTGCGCAATGGACGGGGGGGGTAGGAGAAACAGAAGCATCATCTTCATC670.6838828212718179No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGAT630.6430539961212617No Hit
ATTGCGCAATGCGAGGCACGGGGGGGGTAGGAGAAACAGAAGCATCATCT580.5920179646830662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG480.48994590180667547No Hit
ATTGCGCAATGTTTATGCGGGGAAGAGCTCGTAGGCGGTTTGTCGCGTCT390.39808104521792387No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC380.38787383893028476No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC370.3776666326426457No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGAT370.3776666326426457No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA360.36745942635500667No Hit
ATTGCGCAATGGCGTGGGGGGGGCCCCTCCTTGTCATGTCTGCCGGCATG300.30621618862917216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT280.28580177605389406No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT270.27559456976625496No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT260.2653873634786159No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA240.24497295090333773No Hit
ATTGCGCAATGGTACAAGGGGGGAGCCCGATCTCAGCGCCGCGCCCGACG220.2245585383280596No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT210.21435133204042053No Hit
ATTGCGCAATGAAACGCCCGGGAAGTGAAGAAGGGCGCACGGTGAATGCC200.20414412575278149No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG190.19393691946514238No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCT180.18372971317750333No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG170.17352250688986423No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG160.16331530060222518No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC160.16331530060222518No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAGAGATGATCG160.16331530060222518No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGA150.15310809431458608No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.15310809431458608No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA150.15310809431458608No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA140.14290088802694703No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAG140.14290088802694703No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT130.13269368173930796No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG130.13269368173930796No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGAGAGATGATCG120.12248647545166887No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12248647545166887No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC120.12248647545166887No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC110.1122792691640298No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA110.1122792691640298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT100.10207206287639074No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGCTGTCTCTTAT100.10207206287639074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)